Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution

Yahya Açil; Jürgen Brinckmann; Peter Behrens; Peter K. Müller; Boris Bätge

doi:10.1016/S0021-9673(96)00729-7

Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution

Yahya Açil^*, Jürgen Brinckmann, Peter Behrens, Peter K. Müller, Boris Bätge

^*Corresponding author for this work

12 Citations (Scopus)

Abstract

A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.

Original language	English
Journal	Journal of Chromatography A
Volume	758
Issue number	2
Pages (from-to)	313-318
Number of pages	6
ISSN	0021-9673
DOIs	https://doi.org/10.1016/S0021-9673(96)00729-7
Publication status	Published - 17.01.1997

Funding

This work was supportedb y DFG (Deutsche For-schungsgemeinschaSftF;B 367-A1 and Br 1146/2-1), by a grant from the BMFT (Bundesministerium ftir Forschung und Technologie0, 1 KM 9303) and by a grant from the registereds ociety for investigation and fight of rheumatoid diseases (Bad BramstedtG, ermany).

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0021-9673(96)00729-7

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abstract = "A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.",

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AU - Açil, Yahya

AU - Brinckmann, Jürgen

AU - Behrens, Peter

AU - Müller, Peter K.

AU - Bätge, Boris

PY - 1997/1/17

Y1 - 1997/1/17

N2 - A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.

AB - A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.

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Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution

Abstract

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