TY - JOUR
T1 - Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution
AU - Açil, Yahya
AU - Brinckmann, Jürgen
AU - Behrens, Peter
AU - Müller, Peter K.
AU - Bätge, Boris
PY - 1997/1/17
Y1 - 1997/1/17
N2 - A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.
AB - A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.
UR - http://www.scopus.com/inward/record.url?scp=0031575377&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(96)00729-7
DO - 10.1016/S0021-9673(96)00729-7
M3 - Journal articles
C2 - 9042738
AN - SCOPUS:0031575377
SN - 0021-9673
VL - 758
SP - 313
EP - 318
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -
