Abstract
Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is a widely used technology platform for diagnostic biomarker discovery in tissue, plasma and serum. High-throughput and simplicity of experimental procedures have allowed this technology to become an important research tool for biomarker discovery during the last years. This review provides an overview of SELDI-TOF functionality, its advantages and drawbacks and gives a current literature overview of colorectal cancer based serum biomarker detection. Further improvements in instrumentation sensitivity and labelling chemistries will enable detection of novel, tissue-leakage biomarkers in serum. However, major emphasis should be given on subsequent identification of differentially observed protein peaks detected by SELDI-TOF. Clinical validation in large patient cohorts will then allow transferring novel biomarkers into clinical use.
| Original language | English |
|---|---|
| Journal | Archives of Physiology and Biochemistry |
| Volume | 116 |
| Issue number | 4-5 |
| Pages (from-to) | 188-196 |
| Number of pages | 9 |
| ISSN | 1381-3455 |
| DOIs | |
| Publication status | Published - 01.12.2010 |
Funding
Grants from the Werner and Klara Kreitz Foundation (Kiel, Schleswig-Holstein), the H.W. & J. Hector-Foundation (Weinheim), the Ad Infinitum Foundation (Lübeck), the Swedish Cancer Society (Cancerfonden), the Cancer Society Stockholm (Cancerföreningen) and the Swedish Research Council are gratefully acknowledged.
