TY - JOUR
T1 - SELDI-TOF serum proteomics and colorectal cancer: A current overview
AU - Gemoll, Timo
AU - Roblick, Uwe Johannes
AU - Auer, Gert
AU - Jörnvall, Hans
AU - Habermann, Jens Karsten
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is a widely used technology platform for diagnostic biomarker discovery in tissue, plasma and serum. High-throughput and simplicity of experimental procedures have allowed this technology to become an important research tool for biomarker discovery during the last years. This review provides an overview of SELDI-TOF functionality, its advantages and drawbacks and gives a current literature overview of colorectal cancer based serum biomarker detection. Further improvements in instrumentation sensitivity and labelling chemistries will enable detection of novel, tissue-leakage biomarkers in serum. However, major emphasis should be given on subsequent identification of differentially observed protein peaks detected by SELDI-TOF. Clinical validation in large patient cohorts will then allow transferring novel biomarkers into clinical use.
AB - Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is a widely used technology platform for diagnostic biomarker discovery in tissue, plasma and serum. High-throughput and simplicity of experimental procedures have allowed this technology to become an important research tool for biomarker discovery during the last years. This review provides an overview of SELDI-TOF functionality, its advantages and drawbacks and gives a current literature overview of colorectal cancer based serum biomarker detection. Further improvements in instrumentation sensitivity and labelling chemistries will enable detection of novel, tissue-leakage biomarkers in serum. However, major emphasis should be given on subsequent identification of differentially observed protein peaks detected by SELDI-TOF. Clinical validation in large patient cohorts will then allow transferring novel biomarkers into clinical use.
UR - http://www.scopus.com/inward/record.url?scp=78649270438&partnerID=8YFLogxK
U2 - 10.3109/13813455.2010.495130
DO - 10.3109/13813455.2010.495130
M3 - Scientific review articles
C2 - 20615064
AN - SCOPUS:78649270438
SN - 1381-3455
VL - 116
SP - 188
EP - 196
JO - Archives of Physiology and Biochemistry
JF - Archives of Physiology and Biochemistry
IS - 4-5
ER -
