Role of cytochrome P450 in the control of the production of erythropoietin

J. Fandrey; F. P. Seydel; C. P. Siegers; W. Jelkmann

doi:10.1016/0024-3205(90)90225-G

Role of cytochrome P₄₅₀ in the control of the production of erythropoietin

J. Fandrey^*, F. P. Seydel, C. P. Siegers, W. Jelkmann

^*Corresponding author for this work

Institute of Physiology

46 Citations (Scopus)

Abstract

Effects of agents affecting cytochrome P_{4 5 0} were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P_{4 5 0}, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T₃ and T₄ stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P_{4 5 0}. These findings support the idea that O₂ sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

Original language	English
Journal	Life Sciences
Volume	47
Issue number	2
Pages (from-to)	127-134
Number of pages	8
ISSN	0024-3205
DOIs	https://doi.org/10.1016/0024-3205(90)90225-G
Publication status	Published - 1990

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0024-3205(90)90225-G

Cite this

@article{0c3d69b1eb9449488fe0799fb00e2180,

title = "Role of cytochrome P450 in the control of the production of erythropoietin",

abstract = "Effects of agents affecting cytochrome P4 5 0 were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P4 5 0, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T3 and T4 stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P4 5 0. These findings support the idea that O2 sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.",

author = "J. Fandrey and Seydel, {F. P.} and Siegers, {C. P.} and W. Jelkmann",

note = "Funding Information: The authors gratefully acknowledge the expert technical assistance of Miss Sabine GieSelmann and the secreterial work of Mrs. Gisela Thaler. This work was financially supported by the Deutsche Forschungsgemeinschaft (DFG-grant Je 95/6-2). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1990",

doi = "10.1016/0024-3205(90)90225-G",

language = "English",

volume = "47",

pages = "127--134",

journal = "Life Sciences",

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TY - JOUR

T1 - Role of cytochrome P450 in the control of the production of erythropoietin

AU - Fandrey, J.

AU - Seydel, F. P.

AU - Siegers, C. P.

AU - Jelkmann, W.

N1 - Funding Information: The authors gratefully acknowledge the expert technical assistance of Miss Sabine GieSelmann and the secreterial work of Mrs. Gisela Thaler. This work was financially supported by the Deutsche Forschungsgemeinschaft (DFG-grant Je 95/6-2). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1990

Y1 - 1990

N2 - Effects of agents affecting cytochrome P4 5 0 were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P4 5 0, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T3 and T4 stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P4 5 0. These findings support the idea that O2 sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

AB - Effects of agents affecting cytochrome P4 5 0 were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P4 5 0, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T3 and T4 stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P4 5 0. These findings support the idea that O2 sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

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