RNA self-processing: Formation of cyclic species and concatemers from a small engineered RNA

Sonja Petkovic, Sabine Müller*

*Corresponding author for this work
10 Citations (Scopus)

Abstract

We have engineered a self-processing RNA, derived from the hairpin ribozyme that runs through a cascade of cleavage and ligation reactions thereby changing its topology. The first two cleavage events leave the resulting RNA with a 5′-OH group and a 2′,3′-cyclic phosphate. Thus, upon refolding, intramolecular ligation delivers a cyclic species. In addition, we demonstrate formation of concatemers resulting from multiple intermolecular ligations. Our results demonstrate the potential of RNA for self-supported topology changes and support the suggestion of 2′,3′-cyclic phosphates being suitable activated building blocks for reversible phosphodiester bond formation in the RNA world.

Original languageEnglish
JournalFEBS Letters
Volume587
Issue number15
Pages (from-to)2435-2440
Number of pages6
ISSN0014-5793
DOIs
Publication statusPublished - 02.08.2013

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