Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches

The 'Adeno-Virus-Enhanced-Receptor-Mediated' (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced. Methods: Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reporter gene for luciferase with either AVET or different liposoms in vitro. Results: In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied. Conclusions: AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.