Relationship between sperm DNA damage, induced acrosome reaction and viability in ICSI patients

B. Ozmen, G. S. Caglar, F. Koster, B. Schopper, K. Diedrich, S. Al-Hasani*

*Corresponding author for this work
    42 Citations (Scopus)


    The DNA damage in human spermatozoa is a relevant predictor of prognosis in male infertility, whereby increased sperm DNA damage impairs the outcomes of artificial reproduction. Theoretically, DNA damage should alter the special cellular functions of human spermatozoa, and lead to diminished acrosome reaction with reduced fertilization rates. Nevertheless, intracytoplasmic sperm injection (ICSI) has been reported to alleviate such negative outcomes due to DNA damage. This study investigated the relationship between DNA fragmentation and acrosome reaction as well as viability in ICSI patients. The study enrolled 42 men undergoing ICSI due to poor sperm parameters. The DNA fragmentation indexes (DFI) were 4-10% in 38% of the cases, and ≥10% in 19% of the cases. The results of both acrosome reaction and viability assays showed negative correlations with DFI values in all cases and especially in cases with fertilization rates <60% (P < 0.05). However, such correlations were not found in cases with fertilization rates >60%. There were no live deliveries in patients with high DFI levels (>10%). In conclusion, negative correlations were identified between increased DNA damage, and acrosome reaction and/or viability of human spermatozoa, especially in cases with reduced fertilization rates.

    Original languageEnglish
    Article number2729
    JournalReproductive BioMedicine Online
    Issue number2
    Pages (from-to)208-214
    Number of pages7
    Publication statusPublished - 08.2007

    Research Areas and Centers

    • Centers: Center for Pre-Implantation Diagnostics (PID)


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