Rearrangement of viral sequences in cytopathogenic pestiviruses

G Meyers; N Tautz; R Stark; J Brownlie; E J Dubovi; M S Collett; H J Thiel

doi:10.1016/0042-6822(92)90199-y

Rearrangement of viral sequences in cytopathogenic pestiviruses

G Meyers, N Tautz, R Stark, J Brownlie, E J Dubovi, M S Collett, H J Thiel

Institute of Virology and Cell Biology

Abstract

Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions of their genomic RNAs were found to be duplicated and rearranged. The viral genomes contain a small duplicated element (SD) derived from the genomic 5' end far downstream of its original context. This sequence is followed by a larger duplication which encompasses the region coding for the protein p80(LD), a molecular marker for cpBVDV. The SD element codes for the viral protease p20. In the case of the viruses analyzed here the aminoterminus of p80 is generated by autoproteolytic removal of the preceding SD-encoded protease. For one of the cpBVDV isolates a specific fusion protein (p28) could be identified which is composed of p20 and part of p10, another viral protein. Molecular characterization of the respective noncytopathogenic counterpart revealed that duplication and rearrangement of sequences as well as the expression of p28 and p80 are specific for the cytopathogenic virus.

Original language	English
Journal	Virology
Volume	191
Issue number	1
Pages (from-to)	368-86
Number of pages	19
ISSN	0042-6822
DOIs	https://doi.org/10.1016/0042-6822(92)90199-y
Publication status	Published - 11.1992

DFG Research Classification Scheme

204-04 Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0042-6822(92)90199-y

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title = "Rearrangement of viral sequences in cytopathogenic pestiviruses",

abstract = "Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions of their genomic RNAs were found to be duplicated and rearranged. The viral genomes contain a small duplicated element (SD) derived from the genomic 5' end far downstream of its original context. This sequence is followed by a larger duplication which encompasses the region coding for the protein p80(LD), a molecular marker for cpBVDV. The SD element codes for the viral protease p20. In the case of the viruses analyzed here the aminoterminus of p80 is generated by autoproteolytic removal of the preceding SD-encoded protease. For one of the cpBVDV isolates a specific fusion protein (p28) could be identified which is composed of p20 and part of p10, another viral protein. Molecular characterization of the respective noncytopathogenic counterpart revealed that duplication and rearrangement of sequences as well as the expression of p28 and p80 are specific for the cytopathogenic virus.",

author = "G Meyers and N Tautz and R Stark and J Brownlie and Dubovi, {E J} and Collett, {M S} and Thiel, {H J}",

year = "1992",

month = nov,

doi = "10.1016/0042-6822(92)90199-y",

language = "English",

volume = "191",

pages = "368--86",

journal = "Virology",

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T1 - Rearrangement of viral sequences in cytopathogenic pestiviruses

AU - Meyers, G

AU - Tautz, N

AU - Stark, R

AU - Brownlie, J

AU - Dubovi, E J

AU - Collett, M S

AU - Thiel, H J

PY - 1992/11

Y1 - 1992/11

N2 - Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions of their genomic RNAs were found to be duplicated and rearranged. The viral genomes contain a small duplicated element (SD) derived from the genomic 5' end far downstream of its original context. This sequence is followed by a larger duplication which encompasses the region coding for the protein p80(LD), a molecular marker for cpBVDV. The SD element codes for the viral protease p20. In the case of the viruses analyzed here the aminoterminus of p80 is generated by autoproteolytic removal of the preceding SD-encoded protease. For one of the cpBVDV isolates a specific fusion protein (p28) could be identified which is composed of p20 and part of p10, another viral protein. Molecular characterization of the respective noncytopathogenic counterpart revealed that duplication and rearrangement of sequences as well as the expression of p28 and p80 are specific for the cytopathogenic virus.

AB - Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions of their genomic RNAs were found to be duplicated and rearranged. The viral genomes contain a small duplicated element (SD) derived from the genomic 5' end far downstream of its original context. This sequence is followed by a larger duplication which encompasses the region coding for the protein p80(LD), a molecular marker for cpBVDV. The SD element codes for the viral protease p20. In the case of the viruses analyzed here the aminoterminus of p80 is generated by autoproteolytic removal of the preceding SD-encoded protease. For one of the cpBVDV isolates a specific fusion protein (p28) could be identified which is composed of p20 and part of p10, another viral protein. Molecular characterization of the respective noncytopathogenic counterpart revealed that duplication and rearrangement of sequences as well as the expression of p28 and p80 are specific for the cytopathogenic virus.

U2 - 10.1016/0042-6822(92)90199-y

DO - 10.1016/0042-6822(92)90199-y

M3 - Journal articles

C2 - 1329326

SN - 0042-6822

VL - 191

SP - 368

EP - 386

JO - Virology

JF - Virology

IS - 1

ER -

Rearrangement of viral sequences in cytopathogenic pestiviruses

Abstract

DFG Research Classification Scheme

UN SDGs

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