Abstract
Current metabolomics approaches utilize cellular metabolite extracts, are destructive, and require high cell numbers. We introduce here an approach that enables the monitoring of cellular metabolism at lower cell numbers by observing the consumption/production of different metabolites over several kinetic data points of up to 48 hours. Our approach does not influence cellular viability, as we optimized the cellular matrix in comparison to other materials used in a variety of in-cell NMR spectroscopy experiments. We are able to monitor real-time metabolism of primary patient cells, which are extremely sensitive to external stress. Measurements are set up in an interleaved manner with short acquisition times (approximately 7 minutes per sample), which allows the monitoring of up to 15 patient samples simultaneously. Further, we implemented our approach for performing tracer-based assays. Our approach will be important not only in the metabolomics fields, but also in individualized diagnostics.
| Original language | English |
|---|---|
| Journal | Angewandte Chemie - International Edition |
| Volume | 59 |
| Issue number | 6 |
| Pages (from-to) | 2304-2308 |
| Number of pages | 5 |
| ISSN | 1433-7851 |
| DOIs | |
| Publication status | Published - 03.02.2020 |
Funding
This project was funded by European Union's Horizon 2020 Initial Training Network HaemMetabolome (No 675790) and the Deutsche Forschungsgemeinschaft (SFB815, project A10 (H.S. (Hubert Serve); N.K.). Albrecht Voelklein has kindly taken pictures of NMR tubes and contributed to the Scheme design. I.A. wishes to express his gratitude to Zuhair Shamleh for his longstanding academic support.
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)
