Real-time investigation of dynamic protein crystallization in living cells

R. Schönherr; M. Klinge; J. M. Rudolph; K. Fita; D. Rehders; F. Lübber; S. Schneegans; I. V. Majoul; M. Duszenko; C. Betzel; A. Brandariz-Nuñez; J. Martinez-Costas; R. Duden; L. Redecke

doi:10.1063/1.4921591

Real-time investigation of dynamic protein crystallization in living cells

R. Schönherr, M. Klinge, J. M. Rudolph, K. Fita, D. Rehders, F. Lübber, S. Schneegans, I. V. Majoul, M. Duszenko, C. Betzel, A. Brandariz-Nuñez, J. Martinez-Costas, R. Duden, L. Redecke^*

^*Corresponding author for this work

5 Citations (Scopus)

Abstract

X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.

Original language	English
Article number	041712
Journal	Structural Dynamics
Volume	2
Issue number	4
DOIs	https://doi.org/10.1063/1.4921591
Publication status	Published - 01.01.2015

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title = "Real-time investigation of dynamic protein crystallization in living cells",

abstract = "X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.",

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AU - Schönherr, R.

AU - Klinge, M.

AU - Rudolph, J. M.

AU - Fita, K.

AU - Rehders, D.

AU - Lübber, F.

AU - Schneegans, S.

AU - Majoul, I. V.

AU - Duszenko, M.

AU - Betzel, C.

AU - Brandariz-Nuñez, A.

AU - Martinez-Costas, J.

AU - Duden, R.

AU - Redecke, L.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.

AB - X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.

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DO - 10.1063/1.4921591

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VL - 2

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JF - Structural Dynamics

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Real-time investigation of dynamic protein crystallization in living cells

Abstract

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