TY - JOUR
T1 - Rapid exchange of large numbers of donor- and host leukocytes after human liver transplantation
AU - Heerwagen, Catharina
AU - Schuster, Martin
AU - Bornscheurer, Albrecht
AU - Pape, Lars
AU - Kirchner, Erich
AU - Schlitt, Hans Juergen
AU - Luettig, Birgit
AU - Westermann, J.
N1 - Funding Information:
Acknowledgements We are indebted to Mrs. Johanna Schuler for her skillful technical assistance, to K.-H. Mahr for clinical support, and to R. Pabst for helpful suggestions regarding the manuscript. We would like to thank the nursing staff of the department of anesthesiology, the members of the RTransplantationsbüro Hannover“ and the transplant surgeons of the Medical School Hannover for their cooperation. This study was supported by the Deutsche For-schungsgemeinschaft (We 1175/4–3).
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - After liver transplantation, the release of donor leukocytes into the host and the uptake of host leukocytes by the graft is one of the earliest immunologic interactions between donor and host. Using three-color flow cytometry, these interactions were investigated in eight patients from 5 min-24 h after receiving HLA unmatched liver grafts. Five minutes after reperfusion, 5.0% ± 1.4% of all blood leukocytes in the host were of donor origin, decreasing to 1.1% ± 0.8% after 24 h. Donor granulocytes preferentially disappeared from the host circulation, whereas no differences were found between NK-cells and various B- and T cell subpopulations. Furthermore, host granulocytes were preferentially retained in the donor liver. Thus, despite extensive pre-operative perfusion, more than 109 donor leukocytes quickly leave the liver graft while host granulocytes preferentially accumulate there. A better understanding of the molecular mechanisms mediating these early interactions might help to develop new strategies for diagnosis and therapy of liver graft rejection.
AB - After liver transplantation, the release of donor leukocytes into the host and the uptake of host leukocytes by the graft is one of the earliest immunologic interactions between donor and host. Using three-color flow cytometry, these interactions were investigated in eight patients from 5 min-24 h after receiving HLA unmatched liver grafts. Five minutes after reperfusion, 5.0% ± 1.4% of all blood leukocytes in the host were of donor origin, decreasing to 1.1% ± 0.8% after 24 h. Donor granulocytes preferentially disappeared from the host circulation, whereas no differences were found between NK-cells and various B- and T cell subpopulations. Furthermore, host granulocytes were preferentially retained in the donor liver. Thus, despite extensive pre-operative perfusion, more than 109 donor leukocytes quickly leave the liver graft while host granulocytes preferentially accumulate there. A better understanding of the molecular mechanisms mediating these early interactions might help to develop new strategies for diagnosis and therapy of liver graft rejection.
UR - http://www.scopus.com/inward/record.url?scp=0034845542&partnerID=8YFLogxK
U2 - 10.1007/s001470100323
DO - 10.1007/s001470100323
M3 - Journal articles
C2 - 11512057
AN - SCOPUS:0034845542
SN - 0934-0874
VL - 14
SP - 240
EP - 247
JO - Transplant International
JF - Transplant International
IS - 4
ER -
