Radiation sensitivity of adipose-derived stem cells isolated from breast tissue

Annemarie Baaße; Friederike Machoy; Dajana Juerß; Jana Baake; Felix Stang; Toralf Reimer; Björn Dirk Krapohl; Guido Hildebrandt

doi:10.3390/ijms19071988

Radiation sensitivity of adipose-derived stem cells isolated from breast tissue

Annemarie Baaße^*, Friederike Machoy, Dajana Juerß, Jana Baake, Felix Stang, Toralf Reimer, Björn Dirk Krapohl, Guido Hildebrandt

^*Corresponding author for this work

Clinic of Plastic Surgery

University of Rostock

10 Citations (Scopus)

Abstract

Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology.

Original language	English
Article number	1988
Journal	International Journal of Molecular Sciences
Volume	19
Issue number	7
ISSN	1661-6596
DOIs	https://doi.org/10.3390/ijms19071988
Publication status	Published - 07.07.2018

Funding

Acknowledgments: Human breast tissue from cosmetic reduction mammoplasties was very kindly provided by the Department of Obstetrics and Gynecology, University Medical Center Rostock, the Clinic of Aesthetics of the Academy for Transdermal Delivery Research e.V., Rostock, the St. Marien Hospital, Berlin and the UKSH, Luebeck. We acknowledge financial support by Deutsche Forschungsgemeinschaft and Universität Rostock/Universitätsmedizin Rostock within the funding programme Open Access Publishing.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/ijms19071988

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title = "Radiation sensitivity of adipose-derived stem cells isolated from breast tissue",

abstract = "Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology.",

author = "Annemarie Baa{\ss}e and Friederike Machoy and Dajana Juer{\ss} and Jana Baake and Felix Stang and Toralf Reimer and Krapohl, \{Bj{\"o}rn Dirk\} and Guido Hildebrandt",

note = "Funding Information: Acknowledgments: Human breast tissue from cosmetic reduction mammoplasties was very kindly provided by the Department of Obstetrics and Gynecology, University Medical Center Rostock, the Clinic of Aesthetics of the Academy for Transdermal Delivery Research e.V., Rostock, the St. Marien Hospital, Berlin and the UKSH, Luebeck. We acknowledge financial support by Deutsche Forschungsgemeinschaft and Universit{\"a}t Rostock/Universit{\"a}tsmedizin Rostock within the funding programme Open Access Publishing. Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

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doi = "10.3390/ijms19071988",

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AU - Baaße, Annemarie

AU - Machoy, Friederike

AU - Juerß, Dajana

AU - Baake, Jana

AU - Stang, Felix

AU - Reimer, Toralf

AU - Krapohl, Björn Dirk

AU - Hildebrandt, Guido

N1 - Funding Information: Acknowledgments: Human breast tissue from cosmetic reduction mammoplasties was very kindly provided by the Department of Obstetrics and Gynecology, University Medical Center Rostock, the Clinic of Aesthetics of the Academy for Transdermal Delivery Research e.V., Rostock, the St. Marien Hospital, Berlin and the UKSH, Luebeck. We acknowledge financial support by Deutsche Forschungsgemeinschaft and Universität Rostock/Universitätsmedizin Rostock within the funding programme Open Access Publishing. Publisher Copyright: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2018/7/7

Y1 - 2018/7/7

N2 - Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology.

AB - Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology.

UR - https://www.scopus.com/pages/publications/85049870393

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DO - 10.3390/ijms19071988

M3 - Journal articles

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SN - 1661-6596

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 7

M1 - 1988

ER -

Radiation sensitivity of adipose-derived stem cells isolated from breast tissue

Abstract

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