TY - JOUR
T1 - Radiation sensitivity of adipose-derived stem cells isolated from breast tissue
AU - Baaße, Annemarie
AU - Machoy, Friederike
AU - Juerß, Dajana
AU - Baake, Jana
AU - Stang, Felix
AU - Reimer, Toralf
AU - Krapohl, Björn Dirk
AU - Hildebrandt, Guido
N1 - Funding Information:
Acknowledgments: Human breast tissue from cosmetic reduction mammoplasties was very kindly provided by the Department of Obstetrics and Gynecology, University Medical Center Rostock, the Clinic of Aesthetics of the Academy for Transdermal Delivery Research e.V., Rostock, the St. Marien Hospital, Berlin and the UKSH, Luebeck. We acknowledge financial support by Deutsche Forschungsgemeinschaft and Universität Rostock/Universitätsmedizin Rostock within the funding programme Open Access Publishing.
Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/7/7
Y1 - 2018/7/7
N2 - Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology.
AB - Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology.
UR - http://www.scopus.com/inward/record.url?scp=85049870393&partnerID=8YFLogxK
U2 - 10.3390/ijms19071988
DO - 10.3390/ijms19071988
M3 - Journal articles
C2 - 29986519
AN - SCOPUS:85049870393
SN - 1661-6596
VL - 19
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 7
M1 - 1988
ER -
