Radiation induced G2/M block and apoptosis in two human sarcoma cell lines with different p53 gene status

Matthias Bache; Jürgen Dunst; Peter Würl; Dirk Fröde; Marion Dietzel; Axel Meye; Hannelore Schmidt; Friedrich Wilhelm Rath; Wolfgang Wohlrab; Henning Dralle; Helge Taubert

doi:10.3892/ijo.11.5.993

Radiation induced G2/M block and apoptosis in two human sarcoma cell lines with different p53 gene status

Matthias Bache, Jürgen Dunst, Peter Würl, Dirk Fröde, Marion Dietzel, Axel Meye, Hannelore Schmidt, Friedrich Wilhelm Rath, Wolfgang Wohlrab, Henning Dralle, Helge Taubert^*

^*Corresponding author for this work

Clinic of Radiotherapy

Martin Luther University

10 Citations (Scopus)

Abstract

We investigated a wild-type (wt) p53 rhabdomyosarcoma (A-204) and a mutated (mt p53) undifferentiated sarcoma cell line (US8-93) for their response to X-ray. The observation period was 0 to 96 h after irradiation. Both cell lines showed a strikingly delayed G2/M arrest and an induction of apoptosis after irradiation. Compared with the cell line A-204 (wt p53), the cell line US8-93 (mt p53) revealed a stronger G2/M arrest. In agreement with this, in terms of viability as well as the rate of apoptosis, A-204 (wt p53) showed a stronger response to irradiation than US8-93 (mt p53). We suggest that the different p53 gene status might be the cause for a different response to irradiation.

Original language	English
Journal	International Journal of Oncology
Volume	11
Issue number	5
Pages (from-to)	993-997
Number of pages	5
ISSN	1019-6439
DOIs	https://doi.org/10.3892/ijo.11.5.993
Publication status	Published - 1997

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title = "Radiation induced G2/M block and apoptosis in two human sarcoma cell lines with different p53 gene status",

abstract = "We investigated a wild-type (wt) p53 rhabdomyosarcoma (A-204) and a mutated (mt p53) undifferentiated sarcoma cell line (US8-93) for their response to X-ray. The observation period was 0 to 96 h after irradiation. Both cell lines showed a strikingly delayed G2/M arrest and an induction of apoptosis after irradiation. Compared with the cell line A-204 (wt p53), the cell line US8-93 (mt p53) revealed a stronger G2/M arrest. In agreement with this, in terms of viability as well as the rate of apoptosis, A-204 (wt p53) showed a stronger response to irradiation than US8-93 (mt p53). We suggest that the different p53 gene status might be the cause for a different response to irradiation.",

author = "Matthias Bache and J{\"u}rgen Dunst and Peter W{\"u}rl and Dirk Fr{\"o}de and Marion Dietzel and Axel Meye and Hannelore Schmidt and Rath, {Friedrich Wilhelm} and Wolfgang Wohlrab and Henning Dralle and Helge Taubert",

year = "1997",

doi = "10.3892/ijo.11.5.993",

language = "English",

volume = "11",

pages = "993--997",

journal = "International Journal of Oncology",

issn = "1019-6439",

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TY - JOUR

T1 - Radiation induced G2/M block and apoptosis in two human sarcoma cell lines with different p53 gene status

AU - Bache, Matthias

AU - Dunst, Jürgen

AU - Würl, Peter

AU - Fröde, Dirk

AU - Dietzel, Marion

AU - Meye, Axel

AU - Schmidt, Hannelore

AU - Rath, Friedrich Wilhelm

AU - Wohlrab, Wolfgang

AU - Dralle, Henning

AU - Taubert, Helge

PY - 1997

Y1 - 1997

N2 - We investigated a wild-type (wt) p53 rhabdomyosarcoma (A-204) and a mutated (mt p53) undifferentiated sarcoma cell line (US8-93) for their response to X-ray. The observation period was 0 to 96 h after irradiation. Both cell lines showed a strikingly delayed G2/M arrest and an induction of apoptosis after irradiation. Compared with the cell line A-204 (wt p53), the cell line US8-93 (mt p53) revealed a stronger G2/M arrest. In agreement with this, in terms of viability as well as the rate of apoptosis, A-204 (wt p53) showed a stronger response to irradiation than US8-93 (mt p53). We suggest that the different p53 gene status might be the cause for a different response to irradiation.

AB - We investigated a wild-type (wt) p53 rhabdomyosarcoma (A-204) and a mutated (mt p53) undifferentiated sarcoma cell line (US8-93) for their response to X-ray. The observation period was 0 to 96 h after irradiation. Both cell lines showed a strikingly delayed G2/M arrest and an induction of apoptosis after irradiation. Compared with the cell line A-204 (wt p53), the cell line US8-93 (mt p53) revealed a stronger G2/M arrest. In agreement with this, in terms of viability as well as the rate of apoptosis, A-204 (wt p53) showed a stronger response to irradiation than US8-93 (mt p53). We suggest that the different p53 gene status might be the cause for a different response to irradiation.

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U2 - 10.3892/ijo.11.5.993

DO - 10.3892/ijo.11.5.993

M3 - Journal articles

AN - SCOPUS:12644315018

SN - 1019-6439

VL - 11

SP - 993

EP - 997

JO - International Journal of Oncology

JF - International Journal of Oncology

IS - 5

ER -

Radiation induced G2/M block and apoptosis in two human sarcoma cell lines with different p53 gene status

Abstract

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