Protein profiling of genomic instability in endometrial cancer

Timo Gemoll, Jens K. Habermann, Johanna Lahmann, Silke Szymczak, Caroline Lundgren, Nana K. Bündgen, Thomas Jungbluth, Britta Nordström, Susanne Becker, Marta I. Lomnytska, Hans Peter Bruch, Andreas Ziegler, Ulf Hellman, Gert Auer, Uwe J. Roblick, Hans Jörnvall*

*Corresponding author for this work
8 Citations (Scopus)


DNA aneuploidy has been identified as a prognostic factor in the majority of epithelial malignancies. We aimed at identifying ploidy-associated protein expression in endometrial cancer of different prognostic subgroups. Comparison of gel electrophoresis-based protein expression patterns between normal endometrium (n = 5), diploid (n = 7), and aneuploid (n = 7) endometrial carcinoma detected 121 ploidy-associated protein forms, 42 differentially expressed between normal endometrium and diploid endometrioid carcinomas, 37 between diploid and aneuploid endometrioid carcinomas, and 41 between diploid endometrioid and aneuploid uterine papillary serous cancer. Proteins were identified by mass spectrometry and evaluated by Ingenuity Pathway Analysis. Targets were confirmed by liquid chromatography/mass spectrometry. Mass spectrometry identified 41 distinct polypeptides and pathway analysis resulted in high-ranked networks with vimentin and Nf-κB as central nodes. These results identify ploidy-associated protein expression differences that overrule histopathology-associated expression differences and emphasize particular protein networks in genomic stability of endometrial cancer.

Original languageEnglish
JournalCellular and Molecular Life Sciences
Issue number2
Pages (from-to)325-333
Number of pages9
Publication statusPublished - 01.01.2012


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