TY - JOUR
T1 - Protein-profiling identified potential biomarkers for the diagnosis of osteitis/osteomyelitis
AU - T., Gemoll
AU - C., Grimme
AU - K., Seide
AU - J.K., Habermann
PY - 2014
Y1 - 2014
N2 - Background: A precise preoperative diagnosis of osteomyelitis is difficult and postoperative bacteriological and histopathological analyses are confirmatory. We therefore aimed at identifying differentially expressed proteins in the serum and tissue that distinguish healthy controls from acutely infected and chronic osteomyelitis. Such markers might serve for innovative early detection and could guide therapeutic intervention. Methods:We compared multiplex-fluorescence two-dimensional gel electrophoresis (2-DE)-based protein expression pattern of serum and tissue between healthy controls (n=9) and patients with acute (n=9) and chronic (n=9) osteomyelitis. 2-DE results were correlated by screening >9,000 serum auto-antibodies of the same patient cohort. Differences in expression levels between the three groups were statistically analyzed including principal component analysis (PCA). Results: Comparing the tissue-proteome of healthy controls and patients with acute and no chronic osteomyelitis (fold-change <2.0 & p <0.05), 105 differentially expressed proteins could be detected. Of those, 15 polypeptides were distinctly expressed between acute and chronic osteomyelitis. Interestingly, serum-based protein-profiling revealed a decreased similarity in protein expression - proportional to other twogroup comparisons - when comparing acute and chronic osteomyelitis. A PCA-based cluster analysis showed clustering and good separation between groups. Conclusion: This study showed significant differences in protein expression of osteomyelitis from healthy controls and from patients with acute and chronic inflammation. Targets will be identified by mass spectrometry and further validated for their biomarker potential on large patient cohorts.
AB - Background: A precise preoperative diagnosis of osteomyelitis is difficult and postoperative bacteriological and histopathological analyses are confirmatory. We therefore aimed at identifying differentially expressed proteins in the serum and tissue that distinguish healthy controls from acutely infected and chronic osteomyelitis. Such markers might serve for innovative early detection and could guide therapeutic intervention. Methods:We compared multiplex-fluorescence two-dimensional gel electrophoresis (2-DE)-based protein expression pattern of serum and tissue between healthy controls (n=9) and patients with acute (n=9) and chronic (n=9) osteomyelitis. 2-DE results were correlated by screening >9,000 serum auto-antibodies of the same patient cohort. Differences in expression levels between the three groups were statistically analyzed including principal component analysis (PCA). Results: Comparing the tissue-proteome of healthy controls and patients with acute and no chronic osteomyelitis (fold-change <2.0 & p <0.05), 105 differentially expressed proteins could be detected. Of those, 15 polypeptides were distinctly expressed between acute and chronic osteomyelitis. Interestingly, serum-based protein-profiling revealed a decreased similarity in protein expression - proportional to other twogroup comparisons - when comparing acute and chronic osteomyelitis. A PCA-based cluster analysis showed clustering and good separation between groups. Conclusion: This study showed significant differences in protein expression of osteomyelitis from healthy controls and from patients with acute and chronic inflammation. Targets will be identified by mass spectrometry and further validated for their biomarker potential on large patient cohorts.
UR - http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L71435083
http://dx.doi.org/10.1007/s00423-014-1175-9
http://sfx.library.uu.nl/utrecht?sid=EMBASE&issn=14352443&id=doi:10.1007%2Fs00423-014-1175-9&atitle=Protein-profiling+identified
UR - http://www.mendeley.com/research/proteinprofiling-identified-potential-biomarkers-diagnosis-osteitisosteomyelitis
U2 - 10.1007/s00423-014-1175-9
DO - 10.1007/s00423-014-1175-9
M3 - Zeitschriftenaufsätze
VL - 399
SP - 382
JO - Langenbeck's Archives of Surgery
JF - Langenbeck's Archives of Surgery
SN - 1435-2443
IS - 3
ER -