Abstract
Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new fish skin cell culture. We further demonstrated that epithelial cells derived from the scales survived in the artificial micro-environment of surrounding fibroblast-like cells. Also, antibody staining indicated that both cell types proliferated and started to build connections with the other cell type. It seems that it is possible to generate an 'artificial skin' with two different cell types. This could lead to the development of a three-dimensional test system, which might be a better in vitro representative of fish skin in vivo than individual skin cell lines.
| Original language | English |
|---|---|
| Journal | European Journal of Cell Biology |
| Volume | 90 |
| Issue number | 12 |
| Pages (from-to) | 1041-1051 |
| Number of pages | 11 |
| ISSN | 0171-9335 |
| DOIs | |
| Publication status | Published - 01.12.2011 |
Funding
The authors would like to thank Jutta Endler for providing the SEM work as well as the Research Institute for Agriculture and Fisheries Mecklenburg-West Pommerania in Born/Darß and the Fischereizucht Reese in Lütjenburg for providing the fish. Writing of this article was made possible in part by grants from the European Fond for Regional Development (EFRE) to Charli Kruse. Appendix A
