TY - JOUR
T1 - Proinflammatory stimuli enhance phagocytosis of apoptotic cells by neutrophil granulocytes
AU - Hellberg, Lars
AU - Fuchs, Sabrina
AU - Gericke, Christoph
AU - Sarkar, Arup
AU - Behnen, Martina
AU - Solbach, Werner
AU - Laskay, Tamás
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Recently, we have reported that, in addition to macrophages, also neutrophil granulocytes can phagocytose apoptotic neutrophils. Based on this finding, we hypothesized that cannibalistic neutrophils at sites of acute infection/inflammation play a major role in the clearance of apoptotic neutrophils. Since at sites of infection/inflammation neutrophils are exposed to microbial constituents and proinflammatory cytokines, in the present study we analyzed the effect of TLR-ligands and cytokines on the ability of neutrophils to phagocytose apoptotic cells in vitro. We observed that exposure to ligands of TLR2 (Malp2, Pam3CSK4), TLR4 (LPS), TLR7/TLR8 (R848), and TLR9 (ODN 2006) led to increased phagocytosis of apoptotic cells by neutrophils. In addition, proinflammatory cytokines such as TNF and GM-CSF strongly enhanced the uptake of apoptotic cells by neutrophils. These results support the hypothesis that neutrophils acquire the ability to phagocytose apoptotic cells at sites of acute infection/inflammation and thereby can contribute to the resolution of inflammation.
AB - Recently, we have reported that, in addition to macrophages, also neutrophil granulocytes can phagocytose apoptotic neutrophils. Based on this finding, we hypothesized that cannibalistic neutrophils at sites of acute infection/inflammation play a major role in the clearance of apoptotic neutrophils. Since at sites of infection/inflammation neutrophils are exposed to microbial constituents and proinflammatory cytokines, in the present study we analyzed the effect of TLR-ligands and cytokines on the ability of neutrophils to phagocytose apoptotic cells in vitro. We observed that exposure to ligands of TLR2 (Malp2, Pam3CSK4), TLR4 (LPS), TLR7/TLR8 (R848), and TLR9 (ODN 2006) led to increased phagocytosis of apoptotic cells by neutrophils. In addition, proinflammatory cytokines such as TNF and GM-CSF strongly enhanced the uptake of apoptotic cells by neutrophils. These results support the hypothesis that neutrophils acquire the ability to phagocytose apoptotic cells at sites of acute infection/inflammation and thereby can contribute to the resolution of inflammation.
UR - http://www.scopus.com/inward/record.url?scp=84855176013&partnerID=8YFLogxK
U2 - 10.1100/2011/413271
DO - 10.1100/2011/413271
M3 - Journal articles
C2 - 22125470
AN - SCOPUS:84855176013
SN - 1537-744X
VL - 11
SP - 2230
EP - 2236
JO - TheScientificWorldJournal
JF - TheScientificWorldJournal
ER -