Production of recombinant antibodies in drosophila melanogaster s2 cells

Daniel X. Johansson*, Thomas Krey, Oskar Andersson

*Corresponding author for this work
9 Citations (Scopus)

Abstract

One of the major bottlenecks in antibody discovery for research and therapeutic applications is the need for large quantities of protein in a short amount of time. Here we describe an alternative method using the Drosophila melanogaster S2 expression system to produce high levels of antibodies (both IgG and Fab) with equivalent binding properties to antibodies produced in mammalian cell expression systems. Using the Drosophila S2 expression system for antibody production has many advantages over current mammalian systems making antibody expression, purification, and evaluation a much less time-consuming process.

Original languageEnglish
Title of host publicationAntibody Engineering : Methods and Protocols, Second Edition
Number of pages12
PublisherHumana Press Inc.
Publication date2012
Pages359-370
ISBN (Print)9781617799730
DOIs
Publication statusPublished - 2012

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