Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain

Niklas Tenhaef, Christian Brüsseler, Andreas Radek, René Hilmes, Pornkamol Unrean, Jan Marienhagen, Stephan Noack*

*Corresponding author for this work


It was found that Corynebacterium glutamicum ΔiolR devoid of the transcriptional regulator IolR accumulates high amounts of d-xylonate when cultivated in the presence of d-xylose. Detailed analyses of constructed deletion mutants revealed that the putative myo-inositol 2-dehydrogenase IolG also acts as d-xylose dehydrogenase and is mainly responsible for d-xylonate oxidation in this organism. Process development for d-xylonate production was initiated by cultivating C. glutamicum ΔiolR on defined d-xylose/d-glucose mixtures under batch and fed-batch conditions. The resulting yield matched the theoretical maximum of 1 mol mol-1 and high volumetric productivities of up to 4 g L-1 h-1 could be achieved. Subsequently, a novel one-pot sequential hydrolysis and fermentation process based on optimized medium containing hydrolyzed sugarcane bagasse was developed. Cost-efficiency and abundance of second-generation substrates, good performance indicators, and enhanced market access using a non-recombinant strain open the perspective for a commercially viable bioprocess for d-xylonate production in the near future.

Original languageEnglish
JournalBioresource technology
Pages (from-to)332-339
Number of pages8
Publication statusPublished - 11.2018
Externally publishedYes

DFG Research Classification Scheme

  • 201-01 Biochemistry


Dive into the research topics of 'Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain'. Together they form a unique fingerprint.

Cite this