Production of coronavirus nonstructural proteins in soluble form for crystallization

Yvonne Piotrowski; Rajesh Ponnusamy; Stephanie Glaser; Anniken Daabach; Ralf Moll; Rolf Hilgenfeld

doi:10.1007/978-1-59745-181-9_13

Production of coronavirus nonstructural proteins in soluble form for crystallization

Yvonne Piotrowski^*, Rajesh Ponnusamy, Stephanie Glaser, Anniken Daabach, Ralf Moll, Rolf Hilgenfeld

^*Corresponding author for this work

Institute of Biochemistry

2 Citations (Scopus)

Abstract

For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.

Original language	English
Title of host publication	SARS- and Other Coronaviruses : Laboratory Protocols
Number of pages	21
Publisher	Humana Press Inc.
Publication date	01.01.2008
Pages	139-159
ISBN (Print)	9781588298676
DOIs	https://doi.org/10.1007/978-1-59745-181-9_13
Publication status	Published - 01.01.2008

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

Coronavirus related work

Research on SARS-CoV-2 / COVID-19

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10.1007/978-1-59745-181-9_13

Cite this

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abstract = "For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.",

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language = "English",

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Production of coronavirus nonstructural proteins in soluble form for crystallization. / Piotrowski, Yvonne; Ponnusamy, Rajesh; Glaser, Stephanie et al.
SARS- and Other Coronaviruses: Laboratory Protocols. Humana Press Inc., 2008. p. 139-159 (Methods in Molecular Biology; Vol. 454).

Research output: Chapters in Books/Reports/Conference Proceedings › Chapter › peer-review

TY - CHAP

T1 - Production of coronavirus nonstructural proteins in soluble form for crystallization

AU - Piotrowski, Yvonne

AU - Ponnusamy, Rajesh

AU - Glaser, Stephanie

AU - Daabach, Anniken

AU - Moll, Ralf

AU - Hilgenfeld, Rolf

PY - 2008/1/1

Y1 - 2008/1/1

N2 - For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.

AB - For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.

UR - https://www.scopus.com/pages/publications/84934441385

U2 - 10.1007/978-1-59745-181-9_13

DO - 10.1007/978-1-59745-181-9_13

M3 - Chapter

C2 - 19057878

AN - SCOPUS:84934441385

SN - 9781588298676

T3 - Methods in Molecular Biology

SP - 139

EP - 159

BT - SARS- and Other Coronaviruses

PB - Humana Press Inc.

ER -

Production of coronavirus nonstructural proteins in soluble form for crystallization

Abstract

UN SDGs

Research Areas and Centers

Coronavirus related work

Access to Document

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