Production of coronavirus nonstructural proteins in soluble form for crystallization

Yvonne Piotrowski*, Rajesh Ponnusamy, Stephanie Glaser, Anniken Daabach, Ralf Moll, Rolf Hilgenfeld

*Corresponding author for this work
1 Citation (Scopus)


For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.

Original languageEnglish
Title of host publicationSARS- and Other Coronaviruses : Laboratory Protocols
Number of pages21
PublisherHumana Press Inc.
Publication date01.01.2008
ISBN (Print)9781588298676
Publication statusPublished - 01.01.2008

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

Coronavirus related work

  • Research on SARS-CoV-2 / COVID-19


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