Abstract
Embryonic germ (EG) cells of line EG-1 derived from mouse primordial germ cells were investigated for their in vitro differentiation capacity. By cultivation as embryo-like aggregates EG-1 cells differentiated into cardiac, skeletal muscle and neuronal cells accompanied by the expression of tissue-specific genes and proteins as shown by RT-PCR analysis and indirect immunofluorescence. In comparison to embryonic stem (ES) cells of line D3 the efficiency of differentiation into cardiac and muscle cells was comparatively low, whereas spontaneous neuronal differentiation was more efficient than in D3 cells. Furthermore, the distribution of cell cycle phases as a parameter for the differentiation state was analysed in undifferentiated EG cells and ES cells and compared to data obtained for embryonic carcinoma (EC) cells of line P19 and differentiated, epithelioid EPI-7 cells. Flow cytometric analysis revealed similar cell cycle phase distributions in EG, EC and ES cells. In contrast, the somatic differentiated EPI-7 cells showed a longer G1-phase and shorter S- and G2/M-phases. Together, our results demonstrate that the differentiation state and capacity of EG cells in vitro resemble that of totipotent ES cells.
| Original language | English |
|---|---|
| Journal | Cell Biology International |
| Volume | 20 |
| Issue number | 8 |
| Pages (from-to) | 579-587 |
| Number of pages | 9 |
| ISSN | 1065-6995 |
| DOIs | |
| Publication status | Published - 01.01.1996 |
Funding
The skilful technical assistance of Birgit Adam, Helga Berthold, Karla Meier, Sabine Sommerfeld and Oda Weiß is gratefully acknowledged. The authors wish to thank Dr Colin L. Stewart (Nutley, New Jersey, U.S.A.) for kindly providing EG-1 cells, Dr Christine Mummery (Utrecht, The Netherlands) for EPI-7 cells, Dr Davor Solter for the gift of SSEA-1 antibody and Dr Dieter Fürst for the antibody T12. The results were obtained with financial support of the Bundesministerium für Forschung und Technologie (BMFT, 0310299A), Deutsche Forschungsgemeinschaft (DFG, Wo 503/1-3) and Fonds der Chemischen Industrie.
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)
