Abstract
Stearoyl-coenzyme A desaturase 1 (SCD1) is a key enzyme in lipid metabolism and is expressed in cumulus cells. The objective of the present study was to evaluate the effect of SCD1 inhibition in human cumulus cells on triglyceride content, steroidogenesis, and oocyte in vitro maturation. Human cumulus cells were exposed to SCD1 inhibitor CAY10566 (SCDinhib) alone or in combination with oleic acid in primary culture. The SCDinhib markedly suppressed triglyceride accumulation (−47%, P =.01), aromatase gene expression (−36%, P =.02), and estradiol production (−49%, P =.01) even at a dose not affecting cell viability and apoptosis. Human immature oocytes at the germinal vesicle (GV) stage were cocultured with pretreated cumulus cells. The rate of oocytes reaching the metaphase II stage was significantly lower in coculture with SCDinhib-treated cumulus cells than with control cumulus cells (−18%, P <.01), which recovered by oleic acid supplementation. This finding on in vitro maturation rate was also reproducible with mouse GV oocytes. The results suggest that SCD1 activity is required for cumulus cell lipid storage and steroidogenesis. In addition, oocyte maturation is negatively affected by SCD1 inhibition in cumulus cells, possibly due to a deficient lipid-mediated paracrine support.
| Original language | English |
|---|---|
| Journal | Reproductive Sciences |
| Volume | 25 |
| Issue number | 6 |
| Pages (from-to) | 844-853 |
| Number of pages | 10 |
| ISSN | 1933-7191 |
| DOIs | |
| Publication status | Published - 01.06.2018 |
Funding
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author’s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (#1393-7-28). The authors would like to acknowledge the Women?s Reproductive Health Research Center at Tabriz University of Medical Sciences for providing IVF laboratory facilities. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author?s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (#1393-7-28).
