Pregnancy specific β-1 glycoprotein 1 is expressed in pancreatic ductal adenocarcinoma and its subcellular localization correlates with overall survival

Jasmin H. Shahinian; Hannah Fuellgraf; Stefan Tholen; Justin Mastroianni; Julia Daniela Knopf; Markus Kuehs; Bettina Mayer; Manuel Schlimpert; Birte Kulemann; Simon Kuesters; Jens Hoeppner; Ulrich F. Wellner; Martin Werner; Ulrich T. Hopt; Robert Zeiser; Peter Bronsert; Oliver Schilling

doi:10.7150/jca.15864

Pregnancy specific β-1 glycoprotein 1 is expressed in pancreatic ductal adenocarcinoma and its subcellular localization correlates with overall survival

Jasmin H. Shahinian, Hannah Fuellgraf, Stefan Tholen, Justin Mastroianni, Julia Daniela Knopf, Markus Kuehs, Bettina Mayer, Manuel Schlimpert, Birte Kulemann, Simon Kuesters, Jens Hoeppner, Ulrich F. Wellner, Martin Werner, Ulrich T. Hopt, Robert Zeiser, Peter Bronsert, Oliver Schilling^*

^*Corresponding author for this work

Clinic of Surgery

Abstract

Proteins of the pregnancy specific β-1 glycoprotein (PSG) family are renowned for their elevated expression during pregnancy. Only few reports have investigated their expression in adenocarcinomas. We studied the expression of PSG1 in pancreatic adenocarcinoma (PDAC). In a cohort of 104 patient samples, immunohistochemical analysis determined PSG1 expression in every specimen. PSG1 was found at apical and cytoplasmic localization or solely at cytoplasmic localization, with the latter case being correlated to shortened median survival (25 vs 11 months, logrank p-value < 0.001). At the same time, enzyme linked immunosorbent assay (ELISA) did not detect elevated PSG1 levels in the plasma of PDAC patients as opposed to the plasma of healthy, non-pregnant control individuals. We also probed the impact of PSG1 expression in a murine tumor model system, using subcutaneous injection of Colo-26 cells into immunocompetent BALB/c mice. Here, tumor growth was not affected by the expression of human PSG1. Our study reaffirms interest into the tumor-contextual biology of PSG proteins.

Original language	English
Journal	Journal of Cancer
Volume	7
Issue number	14
Pages (from-to)	2018-2027
Number of pages	10
ISSN	0007-0920
DOIs	https://doi.org/10.7150/jca.15864
Publication status	Published - 01.01.2016

Funding

We thank the SFB50 Z1 project members Prof. Dr. Silke Lassmann and Nicola Bittermann for initial establishment of stainings. This study was funded by the Deutsche Forschungsgemeinschaft (SCHI 871/2 and SCHI 871/5, SCHI 871/6, GR 1748/6, INST 39/900-1 and SFB850-Project B8 to O. S., WE 5085/1-1 to U. W.), the European Research Council (ERC-2011-StG 282111-ProteaSys to O.S.), and the Excellence Initiative of the German Federal and State Governments (EXC 294, BIOSS to O.S.). The article processing charge was funded by the German Research Foundation (DFG) and the University of Freiburg in the funding programme Open Access Publishing.

Research Areas and Centers

Research Area: Luebeck Integrated Oncology Network (LION)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.7150/jca.15864

Cite this

Shahinian, J. H., Fuellgraf, H., Tholen, S., Mastroianni, J., Knopf, J. D., Kuehs, M., Mayer, B., Schlimpert, M., Kulemann, B., Kuesters, S., Hoeppner, J., Wellner, U. F., Werner, M., Hopt, U. T., Zeiser, R., Bronsert, P., & Schilling, O. (2016). Pregnancy specific β-1 glycoprotein 1 is expressed in pancreatic ductal adenocarcinoma and its subcellular localization correlates with overall survival. Journal of Cancer, 7(14), 2018-2027. https://doi.org/10.7150/jca.15864

@article{bd6b5cd615f342b6b0da81e8a0ded82f,

title = "Pregnancy specific β-1 glycoprotein 1 is expressed in pancreatic ductal adenocarcinoma and its subcellular localization correlates with overall survival",

abstract = "Proteins of the pregnancy specific β-1 glycoprotein (PSG) family are renowned for their elevated expression during pregnancy. Only few reports have investigated their expression in adenocarcinomas. We studied the expression of PSG1 in pancreatic adenocarcinoma (PDAC). In a cohort of 104 patient samples, immunohistochemical analysis determined PSG1 expression in every specimen. PSG1 was found at apical and cytoplasmic localization or solely at cytoplasmic localization, with the latter case being correlated to shortened median survival (25 vs 11 months, logrank p-value < 0.001). At the same time, enzyme linked immunosorbent assay (ELISA) did not detect elevated PSG1 levels in the plasma of PDAC patients as opposed to the plasma of healthy, non-pregnant control individuals. We also probed the impact of PSG1 expression in a murine tumor model system, using subcutaneous injection of Colo-26 cells into immunocompetent BALB/c mice. Here, tumor growth was not affected by the expression of human PSG1. Our study reaffirms interest into the tumor-contextual biology of PSG proteins.",

author = "Shahinian, \{Jasmin H.\} and Hannah Fuellgraf and Stefan Tholen and Justin Mastroianni and Knopf, \{Julia Daniela\} and Markus Kuehs and Bettina Mayer and Manuel Schlimpert and Birte Kulemann and Simon Kuesters and Jens Hoeppner and Wellner, \{Ulrich F.\} and Martin Werner and Hopt, \{Ulrich T.\} and Robert Zeiser and Peter Bronsert and Oliver Schilling",

year = "2016",

month = jan,

day = "1",

doi = "10.7150/jca.15864",

language = "English",

volume = "7",

pages = "2018--2027",

journal = "Journal of Cancer",

issn = "0007-0920",

publisher = "Springer Nature",

number = "14",

}

Shahinian, JH, Fuellgraf, H, Tholen, S, Mastroianni, J, Knopf, JD, Kuehs, M, Mayer, B, Schlimpert, M, Kulemann, B, Kuesters, S, Hoeppner, J, Wellner, UF, Werner, M, Hopt, UT, Zeiser, R, Bronsert, P & Schilling, O 2016, 'Pregnancy specific β-1 glycoprotein 1 is expressed in pancreatic ductal adenocarcinoma and its subcellular localization correlates with overall survival', Journal of Cancer, vol. 7, no. 14, pp. 2018-2027. https://doi.org/10.7150/jca.15864

TY - JOUR

T1 - Pregnancy specific β-1 glycoprotein 1 is expressed in pancreatic ductal adenocarcinoma and its subcellular localization correlates with overall survival

AU - Shahinian, Jasmin H.

AU - Fuellgraf, Hannah

AU - Tholen, Stefan

AU - Mastroianni, Justin

AU - Knopf, Julia Daniela

AU - Kuehs, Markus

AU - Mayer, Bettina

AU - Schlimpert, Manuel

AU - Kulemann, Birte

AU - Kuesters, Simon

AU - Hoeppner, Jens

AU - Wellner, Ulrich F.

AU - Werner, Martin

AU - Hopt, Ulrich T.

AU - Zeiser, Robert

AU - Bronsert, Peter

AU - Schilling, Oliver

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Proteins of the pregnancy specific β-1 glycoprotein (PSG) family are renowned for their elevated expression during pregnancy. Only few reports have investigated their expression in adenocarcinomas. We studied the expression of PSG1 in pancreatic adenocarcinoma (PDAC). In a cohort of 104 patient samples, immunohistochemical analysis determined PSG1 expression in every specimen. PSG1 was found at apical and cytoplasmic localization or solely at cytoplasmic localization, with the latter case being correlated to shortened median survival (25 vs 11 months, logrank p-value < 0.001). At the same time, enzyme linked immunosorbent assay (ELISA) did not detect elevated PSG1 levels in the plasma of PDAC patients as opposed to the plasma of healthy, non-pregnant control individuals. We also probed the impact of PSG1 expression in a murine tumor model system, using subcutaneous injection of Colo-26 cells into immunocompetent BALB/c mice. Here, tumor growth was not affected by the expression of human PSG1. Our study reaffirms interest into the tumor-contextual biology of PSG proteins.

AB - Proteins of the pregnancy specific β-1 glycoprotein (PSG) family are renowned for their elevated expression during pregnancy. Only few reports have investigated their expression in adenocarcinomas. We studied the expression of PSG1 in pancreatic adenocarcinoma (PDAC). In a cohort of 104 patient samples, immunohistochemical analysis determined PSG1 expression in every specimen. PSG1 was found at apical and cytoplasmic localization or solely at cytoplasmic localization, with the latter case being correlated to shortened median survival (25 vs 11 months, logrank p-value < 0.001). At the same time, enzyme linked immunosorbent assay (ELISA) did not detect elevated PSG1 levels in the plasma of PDAC patients as opposed to the plasma of healthy, non-pregnant control individuals. We also probed the impact of PSG1 expression in a murine tumor model system, using subcutaneous injection of Colo-26 cells into immunocompetent BALB/c mice. Here, tumor growth was not affected by the expression of human PSG1. Our study reaffirms interest into the tumor-contextual biology of PSG proteins.

UR - https://www.scopus.com/pages/publications/85003955449

U2 - 10.7150/jca.15864

DO - 10.7150/jca.15864

M3 - Journal articles

AN - SCOPUS:85003955449

SN - 0007-0920

VL - 7

SP - 2018

EP - 2027

JO - Journal of Cancer

JF - Journal of Cancer

IS - 14

ER -

Pregnancy specific β-1 glycoprotein 1 is expressed in pancreatic ductal adenocarcinoma and its subcellular localization correlates with overall survival

Abstract

Funding

Research Areas and Centers

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this