Predicting the orientation of eukaryotic membrane-spanning proteins

E. Hartmann; T. A. Rapoport; H. F. Lodish

doi:10.1073/pnas.86.15.5786

Predicting the orientation of eukaryotic membrane-spanning proteins

E. Hartmann, T. A. Rapoport, H. F. Lodish

Institute of Biology

Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)

450 Citations (Scopus)

Abstract

We have developed a rule to predict the orientation of the first internal signal-anchor sequence in eukaryotic transmembrane proteins synthesized on the rough endoplasmic reticulum. The difference in the charges of the 15 residues flanking the first internal signal-anchor determine its orientation, with the more positive portion facing the cytosol. In proteins that span the membrane more than once, the orientation of all subsequent transmembrane segments would be determined by that of the most N-terminal one.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Issue number	15
Pages (from-to)	5786-5790
Number of pages	5
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.86.15.5786
Publication status	Published - 01.01.1989

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Predicting the orientation of eukaryotic membrane-spanning proteins

Abstract

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