Präparation critical-point-getrockneter Mausembryonen mit einem Laserskalpell

M. Ludwig, L. C. Busch*, K. Jungnickel, A. Vogel

*Corresponding author for this work
2 Citations (Scopus)

Abstract

We investigated the use of a laser scalpel for tissue sparing demonstration of the deep structures of critical point dried specimens. A Nd: YAG-Laser emitted picosecond pulses (wavelength 1064 nm, pulse width 30 ps) at pulse energies varying from 1 μJ to 6 mJ was used. Differences in the effects on sputtered and unsputtered specimens were found. We separate the floor of the mouth and pharyngeal fornix in mouse embryos (10. developmental day). It was concluded the laser scalpel is superior to conventional mechanical dissecting methods when applied to small dried specimens. The advantages and disadvantages of the laser scalpel are discussed.

Original languageEnglish
JournalAnnals of Anatomy
Volume176
Issue number6
Pages (from-to)559-563
Number of pages5
ISSN0940-9602
DOIs
Publication statusPublished - 1994

Research Areas and Centers

  • Academic Focus: Biomedical Engineering

Fingerprint

Dive into the research topics of 'Präparation critical-point-getrockneter Mausembryonen mit einem Laserskalpell'. Together they form a unique fingerprint.

Cite this