Porcine RPE/choroidal explant cultures

Alexa Klettner; Yoko Miura

doi:10.1007/978-1-4939-8669-9_8

Porcine RPE/choroidal explant cultures

Alexa Klettner, Yoko Miura

University of Kiel

8 Citations (Scopus)

Abstract

The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

Translated title of the contribution	Schweine RPE/Choroid Organkultur
Original language	English
Title of host publication	Retinal Degeneration
Number of pages	10
Volume	2019
Publication date	2019
Pages	109-118
DOIs	https://doi.org/10.1007/978-1-4939-8669-9_8
Publication status	Published - 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 9 Industry, Innovation, and Infrastructure

Research Areas and Centers

Academic Focus: Biomedical Engineering

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10.1007/978-1-4939-8669-9_8

Cite this

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title = "Porcine RPE/choroidal explant cultures",

abstract = "The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch{\textquoteright}s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.",

author = "Alexa Klettner and Yoko Miura",

year = "2019",

doi = "10.1007/978-1-4939-8669-9\_8",

language = "English",

volume = "2019",

series = "Methods in Molecular Biology",

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T1 - Porcine RPE/choroidal explant cultures

AU - Klettner, Alexa

AU - Miura, Yoko

PY - 2019

Y1 - 2019

N2 - The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

AB - The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

UR - https://www.scopus.com/pages/publications/85054892587

UR - http://www.mendeley.com/research/porcine-rpechoroidal-explant-cultures

U2 - 10.1007/978-1-4939-8669-9_8

DO - 10.1007/978-1-4939-8669-9_8

M3 - Chapter

VL - 2019

T3 - Methods in Molecular Biology

SP - 109

EP - 118

BT - Retinal Degeneration

ER -

Porcine RPE/choroidal explant cultures

Abstract

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