Porcine RPE/choroidal explant cultures

Alexa Klettner; Yoko Miura

doi:10.1007/978-1-4939-8669-9_8

Porcine RPE/choroidal explant cultures

Alexa Klettner, Yoko Miura

University of Kiel

Abstract

The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

Translated title of the contribution	Schweine RPE/Choroid Organkultur
Original language	English
Title of host publication	Retinal Degeneration
Number of pages	10
Volume	2019
Publication date	2019
Pages	109-118
DOIs	https://doi.org/10.1007/978-1-4939-8669-9_8
Publication status	Published - 2019

Research Areas and Centers

Academic Focus: Biomedical Engineering

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-8669-9_8

Cite this

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abstract = "The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch{\textquoteright}s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.",

author = "Alexa Klettner and Yoko Miura",

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AB - The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

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UR - http://www.mendeley.com/research/porcine-rpechoroidal-explant-cultures

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M3 - Chapter

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T3 - Methods in Molecular Biology

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BT - Retinal Degeneration

ER -

Porcine RPE/choroidal explant cultures

Abstract

Research Areas and Centers

UN SDGs

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