Porcine RPE/choroidal explant cultures

Alexa Klettner, Yoko Miura


The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch’s membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

Translated title of the contributionSchweine RPE/Choroid Organkultur
Original languageEnglish
Title of host publicationRetinal Degeneration
Number of pages10
Publication date2019
Publication statusPublished - 2019

Research Areas and Centers

  • Academic Focus: Biomedical Engineering


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