TY - JOUR
T1 - Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells
AU - Roolf, Catrin
AU - Kretzschmar, Christin
AU - Timmer, Katrin
AU - Sekora, Anett
AU - Knübel, Gudrun
AU - Escobar, Hugo Murua
AU - Fuellen, Georg
AU - Ibrahim, Saleh M.
AU - Tiedge, Markus
AU - Baltrusch, Simone
AU - Müller, Sarah
AU - Köhling, Rüdiger
AU - Junghanss, Christian
PY - 2016/11/1
Y1 - 2016/11/1
N2 - Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.
AB - Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.
UR - http://www.scopus.com/inward/record.url?scp=84994205147&partnerID=8YFLogxK
U2 - 10.21873/invivo.10991
DO - 10.21873/invivo.10991
M3 - Journal articles
C2 - 27815458
AN - SCOPUS:84994205147
SN - 0258-851X
VL - 30
SP - 751
EP - 760
JO - In Vivo
JF - In Vivo
IS - 6
ER -