Polyclonal activation of immunoglobulin secretion without prior DNA synthesis in human B lymphocytes induced by Klebsiella pneumoniae

W. L. Gross; A. Rucks; G. Hahn; U. Ullmann

doi:10.1016/0090-1229(83)90076-4

Polyclonal activation of immunoglobulin secretion without prior DNA synthesis in human B lymphocytes induced by Klebsiella pneumoniae

W. L. Gross^*, A. Rucks, G. Hahn, U. Ullmann

^*Corresponding author for this work

Clinic of Rheumatology

University of Kiel

8 Citations (Scopus)

Abstract

The capacity of various cell preparations of Klebsiella pneumoniae K43 (Klebs) to induce [³H]thymidine uptake and immunoglobulin (Ig) secretion by human mononuclear blood cells (MNC) and their lymphocyte subpopulations was investigated. All Klebs preparations were virtually devoid of mitogenic properties, in contrast to control preparations of pokeweed mitogen (PWM) and group A streptococcal cell membranes (A-ScM). Klebs induced differentiation of B cells into Ig-secreting cells. B-cell populations that were sufficiently depleted of T cells to be unresponsive to A-ScM ("highly purified B cells") showed a marked response to Klebs. Similarly, the number of plaque-forming cells (PFC) in Klebs-driven cultures did not change after restitution of T cells, whereas the presence of restituted T cells augmented the B-cell response to PWM and A-ScM. Radical removal of adherent MNC ("monocytes"), however, completely abrogated the PFC response and [³H]thymidine uptake of both MNC activated by Klebs and MNC activated by PWM or A-ScM.

Original language	English
Journal	Clinical Immunology and Immunopathology
Volume	27
Issue number	2
Pages (from-to)	261-271
Number of pages	11
ISSN	0090-1229
DOIs	https://doi.org/10.1016/0090-1229(83)90076-4
Publication status	Published - 05.1983

Funding

The expert technical assistance of Mrs. Rautmann and Mr. Utecht is gratefully acknowledged. Supported by Grant Gr609/3-3 from the Deutsche Forschungsgemeinschaft and a grant from the Verein zur Forderung der Erforschung und Bekampfung rheumatischer Erkrankungen.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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title = "Polyclonal activation of immunoglobulin secretion without prior DNA synthesis in human B lymphocytes induced by Klebsiella pneumoniae",

abstract = "The capacity of various cell preparations of Klebsiella pneumoniae K43 (Klebs) to induce [3H]thymidine uptake and immunoglobulin (Ig) secretion by human mononuclear blood cells (MNC) and their lymphocyte subpopulations was investigated. All Klebs preparations were virtually devoid of mitogenic properties, in contrast to control preparations of pokeweed mitogen (PWM) and group A streptococcal cell membranes (A-ScM). Klebs induced differentiation of B cells into Ig-secreting cells. B-cell populations that were sufficiently depleted of T cells to be unresponsive to A-ScM ({"}highly purified B cells{"}) showed a marked response to Klebs. Similarly, the number of plaque-forming cells (PFC) in Klebs-driven cultures did not change after restitution of T cells, whereas the presence of restituted T cells augmented the B-cell response to PWM and A-ScM. Radical removal of adherent MNC ({"}monocytes{"}), however, completely abrogated the PFC response and [3H]thymidine uptake of both MNC activated by Klebs and MNC activated by PWM or A-ScM.",

author = "Gross, \{W. L.\} and A. Rucks and G. Hahn and U. Ullmann",

note = "Funding Information: The expert technical assistance of Mrs. Rautmann and Mr. Utecht is gratefully acknowledged. Supported by Grant Gr609/3-3 from the Deutsche Forschungsgemeinschaft and a grant from the Verein zur Forderung der Erforschung und Bekampfung rheumatischer Erkrankungen. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1983",

month = may,

doi = "10.1016/0090-1229(83)90076-4",

language = "English",

volume = "27",

pages = "261--271",

journal = "Clinical Immunology and Immunopathology",

issn = "0090-1229",

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T1 - Polyclonal activation of immunoglobulin secretion without prior DNA synthesis in human B lymphocytes induced by Klebsiella pneumoniae

AU - Gross, W. L.

AU - Rucks, A.

AU - Hahn, G.

AU - Ullmann, U.

N1 - Funding Information: The expert technical assistance of Mrs. Rautmann and Mr. Utecht is gratefully acknowledged. Supported by Grant Gr609/3-3 from the Deutsche Forschungsgemeinschaft and a grant from the Verein zur Forderung der Erforschung und Bekampfung rheumatischer Erkrankungen. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1983/5

Y1 - 1983/5

N2 - The capacity of various cell preparations of Klebsiella pneumoniae K43 (Klebs) to induce [3H]thymidine uptake and immunoglobulin (Ig) secretion by human mononuclear blood cells (MNC) and their lymphocyte subpopulations was investigated. All Klebs preparations were virtually devoid of mitogenic properties, in contrast to control preparations of pokeweed mitogen (PWM) and group A streptococcal cell membranes (A-ScM). Klebs induced differentiation of B cells into Ig-secreting cells. B-cell populations that were sufficiently depleted of T cells to be unresponsive to A-ScM ("highly purified B cells") showed a marked response to Klebs. Similarly, the number of plaque-forming cells (PFC) in Klebs-driven cultures did not change after restitution of T cells, whereas the presence of restituted T cells augmented the B-cell response to PWM and A-ScM. Radical removal of adherent MNC ("monocytes"), however, completely abrogated the PFC response and [3H]thymidine uptake of both MNC activated by Klebs and MNC activated by PWM or A-ScM.

AB - The capacity of various cell preparations of Klebsiella pneumoniae K43 (Klebs) to induce [3H]thymidine uptake and immunoglobulin (Ig) secretion by human mononuclear blood cells (MNC) and their lymphocyte subpopulations was investigated. All Klebs preparations were virtually devoid of mitogenic properties, in contrast to control preparations of pokeweed mitogen (PWM) and group A streptococcal cell membranes (A-ScM). Klebs induced differentiation of B cells into Ig-secreting cells. B-cell populations that were sufficiently depleted of T cells to be unresponsive to A-ScM ("highly purified B cells") showed a marked response to Klebs. Similarly, the number of plaque-forming cells (PFC) in Klebs-driven cultures did not change after restitution of T cells, whereas the presence of restituted T cells augmented the B-cell response to PWM and A-ScM. Radical removal of adherent MNC ("monocytes"), however, completely abrogated the PFC response and [3H]thymidine uptake of both MNC activated by Klebs and MNC activated by PWM or A-ScM.

UR - https://www.scopus.com/pages/publications/0020594287

U2 - 10.1016/0090-1229(83)90076-4

DO - 10.1016/0090-1229(83)90076-4

M3 - Journal articles

C2 - 6191901

AN - SCOPUS:0020594287

SN - 0090-1229

VL - 27

SP - 261

EP - 271

JO - Clinical Immunology and Immunopathology

JF - Clinical Immunology and Immunopathology

IS - 2

ER -

Polyclonal activation of immunoglobulin secretion without prior DNA synthesis in human B lymphocytes induced by Klebsiella pneumoniae

Abstract

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