TY - JOUR
T1 - Polyclonal activation of immunoglobulin secretion without prior DNA synthesis in human B lymphocytes induced by Klebsiella pneumoniae
AU - Gross, W. L.
AU - Rucks, A.
AU - Hahn, G.
AU - Ullmann, U.
N1 - Funding Information:
The expert technical assistance of Mrs. Rautmann and Mr. Utecht is gratefully acknowledged. Supported by Grant Gr609/3-3 from the Deutsche Forschungsgemeinschaft and a grant from the Verein zur Forderung der Erforschung und Bekampfung rheumatischer Erkrankungen.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1983/5
Y1 - 1983/5
N2 - The capacity of various cell preparations of Klebsiella pneumoniae K43 (Klebs) to induce [3H]thymidine uptake and immunoglobulin (Ig) secretion by human mononuclear blood cells (MNC) and their lymphocyte subpopulations was investigated. All Klebs preparations were virtually devoid of mitogenic properties, in contrast to control preparations of pokeweed mitogen (PWM) and group A streptococcal cell membranes (A-ScM). Klebs induced differentiation of B cells into Ig-secreting cells. B-cell populations that were sufficiently depleted of T cells to be unresponsive to A-ScM ("highly purified B cells") showed a marked response to Klebs. Similarly, the number of plaque-forming cells (PFC) in Klebs-driven cultures did not change after restitution of T cells, whereas the presence of restituted T cells augmented the B-cell response to PWM and A-ScM. Radical removal of adherent MNC ("monocytes"), however, completely abrogated the PFC response and [3H]thymidine uptake of both MNC activated by Klebs and MNC activated by PWM or A-ScM.
AB - The capacity of various cell preparations of Klebsiella pneumoniae K43 (Klebs) to induce [3H]thymidine uptake and immunoglobulin (Ig) secretion by human mononuclear blood cells (MNC) and their lymphocyte subpopulations was investigated. All Klebs preparations were virtually devoid of mitogenic properties, in contrast to control preparations of pokeweed mitogen (PWM) and group A streptococcal cell membranes (A-ScM). Klebs induced differentiation of B cells into Ig-secreting cells. B-cell populations that were sufficiently depleted of T cells to be unresponsive to A-ScM ("highly purified B cells") showed a marked response to Klebs. Similarly, the number of plaque-forming cells (PFC) in Klebs-driven cultures did not change after restitution of T cells, whereas the presence of restituted T cells augmented the B-cell response to PWM and A-ScM. Radical removal of adherent MNC ("monocytes"), however, completely abrogated the PFC response and [3H]thymidine uptake of both MNC activated by Klebs and MNC activated by PWM or A-ScM.
UR - http://www.scopus.com/inward/record.url?scp=0020594287&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(83)90076-4
DO - 10.1016/0090-1229(83)90076-4
M3 - Journal articles
C2 - 6191901
AN - SCOPUS:0020594287
SN - 0090-1229
VL - 27
SP - 261
EP - 271
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -
