Phosphorylation of histone H3T6 by PKCΒ i controls demethylation at histone H3K4

Eric Metzger, Axel Imhof, Dharmeshkumar Patel, Philip Kahl, Katrin Hoffmeyer, Nicolaus Friedrichs, Judith M. Müller, Holger Greschik, Jutta Kirfel, Sujuan Ji, Natalia Kunowska, Christian Beisenherz-Huss, Thomas Günther, Reinhard Buettner, Roland Schüle*

*Corresponding author for this work
199 Citations (Scopus)


Demethylation at distinct lysine residues in histone H3 by lysine-specific demethylase 1 (LSD1) causes either gene repression or activation. As a component of co-repressor complexes, LSD1 contributes to target gene repression by removing mono-and dimethyl marks from lysine 4 of histone H3 (H3K4). In contrast, during androgen receptor (AR)-activated gene expression, LSD1 removes mono-and dimethyl marks from lysine 9 of histone H3 (H3K9). Yet, the mechanisms that control this dual specificity of demethylation are unknown. Here we show that phosphorylation of histone H3 at threonine 6 (H3T6) by protein kinase C beta I (PKCΒ I, also known as PRKCΒ) is the key event that prevents LSD1 from demethylating H3K4 during AR-dependent gene activation. In vitro, histone H3 peptides methylated at lysine 4 and phosphorylated at threonine 6 are no longer LSD1 substrates. In vivo, PKCΒ I co-localizes with AR and LSD1 on target gene promoters and phosphorylates H3T6 after androgen-induced gene expression. RNA interference (RNAi)-mediated knockdown of PKCΒ I abrogates H3T6 phosphorylation, enhances demethylation at H3K4, and inhibits AR-dependent transcription. Activation of PKCΒ I requires androgen-dependent recruitment of the gatekeeper kinase protein kinase C (PKC)-related kinase 1 (PRK1). Notably, increased levels of PKCΒ I and phosphorylated H3T6 (H3T6ph) positively correlate with high Gleason scores of prostate carcinomas, and inhibition of PKCΒ I blocks AR-induced tumour cell proliferation in vitro and cancer progression of tumour xenografts in vivo. Together, our data establish that androgen-dependent kinase signalling leads to the writing of the new chromatin mark H3T6ph, which in consequence prevents removal of active methyl marks from H3K4 during AR-stimulated gene expression.

Original languageEnglish
Issue number7289
Pages (from-to)792-796
Number of pages5
Publication statusPublished - 01.04.2010


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