TY - JOUR
T1 - Pharmacological prevention of radiation-induced dry eye-an experimental study in a rabbit model
AU - Beutel, Julia
AU - Schroder, Christina
AU - von Hof, Katharina
AU - Rades, Dirk
AU - Kosmehl, Hartwig
AU - Wedel, Thilo
AU - Sieg, Peter
AU - Geerling, Gerd
AU - Hakim, Samer George
PY - 2007/9/1
Y1 - 2007/9/1
N2 - Purpose: To evaluate the radioprotective effect of lidocaine, amifostine and pilocarpine on lacrimal glands. Methods: Twenty-five rabbits were randomized into five groups: the control, irradiated/sham-treated, irradiated/lidocaine-pretreated, irradiated/amifostine-pretreated and irradiated/pilocarpine-pretreated groups. One week before irradiation, 72 h and 1 month afterward, the inferior lacrimal gland was investigated histomorphologically, immunohistochemically [tenascin-C and alpha smooth muscle actin (α-SMA)] and functionally using scintigraphy and the Schirmer test. Results: Compared with control animals, the lacrimal ejection fraction (LEF) in the irradiated/sham-treated group was significantly reduced 72 h afterwards. Pilocarpine - as well as amifostine-pretreated animals showed a slightly lower reduction. Lidocaine stabilized the LEF. Immunohistochemically, a significant loss of α-SMA and an up-regulation of tenascin-C expression in irradiated/untreated glands were evident. Pretreatment with lidocaine and amifostine-but not with pilocarpine-induced lower up-regulation of TN-C expression 72 h after radiation. One month after irradiation a reduction of the immunhistochemical changes at all was found. Ultrastructural damage was observed in irradiated/non-treated and pilocarpine-pretreated glands, whereas lidocaine and amifostine preserved the ultrastructure. Conclusion: Morphologic and functional findings could prove a prevailing protection profile of amifostine and especially of lidocaine on lacrimal glands. This may provide a prophylactic approach in the radioprotection of the lacrimal glands during radiotherapy of the orbital region.
AB - Purpose: To evaluate the radioprotective effect of lidocaine, amifostine and pilocarpine on lacrimal glands. Methods: Twenty-five rabbits were randomized into five groups: the control, irradiated/sham-treated, irradiated/lidocaine-pretreated, irradiated/amifostine-pretreated and irradiated/pilocarpine-pretreated groups. One week before irradiation, 72 h and 1 month afterward, the inferior lacrimal gland was investigated histomorphologically, immunohistochemically [tenascin-C and alpha smooth muscle actin (α-SMA)] and functionally using scintigraphy and the Schirmer test. Results: Compared with control animals, the lacrimal ejection fraction (LEF) in the irradiated/sham-treated group was significantly reduced 72 h afterwards. Pilocarpine - as well as amifostine-pretreated animals showed a slightly lower reduction. Lidocaine stabilized the LEF. Immunohistochemically, a significant loss of α-SMA and an up-regulation of tenascin-C expression in irradiated/untreated glands were evident. Pretreatment with lidocaine and amifostine-but not with pilocarpine-induced lower up-regulation of TN-C expression 72 h after radiation. One month after irradiation a reduction of the immunhistochemical changes at all was found. Ultrastructural damage was observed in irradiated/non-treated and pilocarpine-pretreated glands, whereas lidocaine and amifostine preserved the ultrastructure. Conclusion: Morphologic and functional findings could prove a prevailing protection profile of amifostine and especially of lidocaine on lacrimal glands. This may provide a prophylactic approach in the radioprotection of the lacrimal glands during radiotherapy of the orbital region.
UR - http://www.scopus.com/inward/record.url?scp=34548010052&partnerID=8YFLogxK
U2 - 10.1007/s00417-007-0548-y
DO - 10.1007/s00417-007-0548-y
M3 - Journal articles
C2 - 17318564
AN - SCOPUS:34548010052
SN - 0721-832X
VL - 245
SP - 1347
EP - 1355
JO - Graefe's Archive for Clinical and Experimental Ophthalmology
JF - Graefe's Archive for Clinical and Experimental Ophthalmology
IS - 9
ER -
