Pericellular PO2 and O2 consumption in monolayer cell cultures

E. Metzen; M. Wolff; J. Fandrey; W. Jelkmann

doi:10.1016/0034-5687(94)00125-J

Pericellular P_O₂ and O₂ consumption in monolayer cell cultures

E. Metzen, M. Wolff, J. Fandrey, W. Jelkmann^*

^*Corresponding author for this work

Institute of Physiology

University of Bonn

126 Citations (Scopus)

Abstract

The current study was based on the uncertainty as to how well monolayer cell cultures growing in customary polystyrene dishes are supplied with O₂. For dishes maintained in an air-5% CO₂ atmosphere at 37°C, microelectrode measurements revealed that the pericellular steady-state P_O₂ was 78 mm Hg in confluent bovine endothelial, 110 mm Hg in rat renal mesangial, and 0 (<0.2) mm Hg in renal (LLC-PK₁ and LLC-MK₂) or hepatic (HepG2, Hep3B) epithelial cell cultures. These measured P_O₂ values were in good agreement with those calculated from Fick's law of gas diffusion, applied for the present culture conditions (one-dimensional O₂ diffusion, 0.52 cm medium height), the individual cell layer density and the tissue-specific rate of O₂ utilization. Our results provide reasons to speculate that conventional monolayer cultures are often hypoxic when incubated in an air-5% CO₂ atmosphere. Diffusion-limitations of cellular O₂ availability are to be taken into consideration when tissue cultures are used to study P_O₂-dependent processes.

Original language	English
Journal	Respiration Physiology
Volume	100
Issue number	2
Pages (from-to)	101-106
Number of pages	6
ISSN	0034-5687
DOIs	https://doi.org/10.1016/0034-5687(94)00125-J
Publication status	Published - 05.1995

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0034-5687(94)00125-J

Cite this

@article{84afc3029e0b48039064c49468479821,

title = "Pericellular PO2 and O2 consumption in monolayer cell cultures",

abstract = "The current study was based on the uncertainty as to how well monolayer cell cultures growing in customary polystyrene dishes are supplied with O2. For dishes maintained in an air-5% CO2 atmosphere at 37°C, microelectrode measurements revealed that the pericellular steady-state PO2 was 78 mm Hg in confluent bovine endothelial, 110 mm Hg in rat renal mesangial, and 0 (<0.2) mm Hg in renal (LLC-PK1 and LLC-MK2) or hepatic (HepG2, Hep3B) epithelial cell cultures. These measured PO2 values were in good agreement with those calculated from Fick's law of gas diffusion, applied for the present culture conditions (one-dimensional O2 diffusion, 0.52 cm medium height), the individual cell layer density and the tissue-specific rate of O2 utilization. Our results provide reasons to speculate that conventional monolayer cultures are often hypoxic when incubated in an air-5% CO2 atmosphere. Diffusion-limitations of cellular O2 availability are to be taken into consideration when tissue cultures are used to study PO2-dependent processes.",

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T1 - Pericellular PO2 and O2 consumption in monolayer cell cultures

AU - Metzen, E.

AU - Wolff, M.

AU - Fandrey, J.

AU - Jelkmann, W.

PY - 1995/5

Y1 - 1995/5

N2 - The current study was based on the uncertainty as to how well monolayer cell cultures growing in customary polystyrene dishes are supplied with O2. For dishes maintained in an air-5% CO2 atmosphere at 37°C, microelectrode measurements revealed that the pericellular steady-state PO2 was 78 mm Hg in confluent bovine endothelial, 110 mm Hg in rat renal mesangial, and 0 (<0.2) mm Hg in renal (LLC-PK1 and LLC-MK2) or hepatic (HepG2, Hep3B) epithelial cell cultures. These measured PO2 values were in good agreement with those calculated from Fick's law of gas diffusion, applied for the present culture conditions (one-dimensional O2 diffusion, 0.52 cm medium height), the individual cell layer density and the tissue-specific rate of O2 utilization. Our results provide reasons to speculate that conventional monolayer cultures are often hypoxic when incubated in an air-5% CO2 atmosphere. Diffusion-limitations of cellular O2 availability are to be taken into consideration when tissue cultures are used to study PO2-dependent processes.

AB - The current study was based on the uncertainty as to how well monolayer cell cultures growing in customary polystyrene dishes are supplied with O2. For dishes maintained in an air-5% CO2 atmosphere at 37°C, microelectrode measurements revealed that the pericellular steady-state PO2 was 78 mm Hg in confluent bovine endothelial, 110 mm Hg in rat renal mesangial, and 0 (<0.2) mm Hg in renal (LLC-PK1 and LLC-MK2) or hepatic (HepG2, Hep3B) epithelial cell cultures. These measured PO2 values were in good agreement with those calculated from Fick's law of gas diffusion, applied for the present culture conditions (one-dimensional O2 diffusion, 0.52 cm medium height), the individual cell layer density and the tissue-specific rate of O2 utilization. Our results provide reasons to speculate that conventional monolayer cultures are often hypoxic when incubated in an air-5% CO2 atmosphere. Diffusion-limitations of cellular O2 availability are to be taken into consideration when tissue cultures are used to study PO2-dependent processes.

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