Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180

Arno Kromminga; Christian Scheckenbach; Matthias Georgi; Christine Hagel; Rüdiger Arndt; Enno Christophers; Eva B. Bröcker; Detlef Zillikens

doi:10.1006/jaut.2000.0437

Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180

Arno Kromminga, Christian Scheckenbach, Matthias Georgi, Christine Hagel, Rüdiger Arndt, Enno Christophers, Eva B. Bröcker, Detlef Zillikens^*

^*Corresponding author for this work

Department of Dermatology, Allergology and Venereology

82 Citations (Scopus)

Abstract

Bullous pemphigoid (BP) and linear IgA disease (LAD) are autoimmune subepidermal blistering skin disease associated with autoantibodies against the transmembrane hemidesmosomal protein BP180/type XVII collagen. It has been demonstrated previously that BP is characterized predominantly by IgG autoantibodies, while autoantibodies in LAD mainly belong to the IgA isotype. The aim of the present study was to investigate the hypothesis that there is a significant overlap in the autoantibody isotype associated with these two disease. Several new recombinant forms of BP180 were generated in the baculovirus expression system, including the full-length protein. IgG autoantibodies to BP 180 were detectable in 39 of 40 (98%) of BP sera; interestingly, 88% of BP sera also contained IgA anti-BP180 autoantibodies. Similarly, anit-BP180 reactivity in LAD sera (n=22) was also attributed to both an IgA (68%) and an IgG (76%) autoanitbody response. IgA and IgG autoantibodies to the intracellular portion of BP180 were found in 14% and 28% of BP sera, respectively, and in 8% of LAD sera (same percentage for both isotypes). Our findings clearly demonstrate that both BP and LAD patients have dual IgA and IgG autoimmune response to BP180 which is directed not only to the ectodomain, but also to the intracellular portion of this protein. (C) 2000 Academic Press.

Original language	English
Journal	Journal of Autoimmunity
Volume	15
Issue number	3
Pages (from-to)	293-300
Number of pages	8
ISSN	0896-8411
DOIs	https://doi.org/10.1006/jaut.2000.0437
Publication status	Published - 2000

Funding

This work has been supported by grant 98.073.1 from the Wilhelm Sander-Stiftung, Munich, Germany (D.Z.). We thank Drs Gerald Messer and Karin Partscht, Munich, Germany, for providing us with serum samples from patients with linear IgA disease and Drs George J. Giudice and Luis A. Diaz, Milwaukee, WI, USA, for providing us with rabbit sera 136 and 594. We are also grateful to Christa Knaus, Würzburg, for assistance producing LAD-1 from conditioned HaCaT cell medium, and Hartmut Wulf, Hamburg, for help preparing the figures.

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10.1006/jaut.2000.0437

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@article{b5b9b01429d747f4a11d6d8a54af7614,

title = "Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180",

abstract = "Bullous pemphigoid (BP) and linear IgA disease (LAD) are autoimmune subepidermal blistering skin disease associated with autoantibodies against the transmembrane hemidesmosomal protein BP180/type XVII collagen. It has been demonstrated previously that BP is characterized predominantly by IgG autoantibodies, while autoantibodies in LAD mainly belong to the IgA isotype. The aim of the present study was to investigate the hypothesis that there is a significant overlap in the autoantibody isotype associated with these two disease. Several new recombinant forms of BP180 were generated in the baculovirus expression system, including the full-length protein. IgG autoantibodies to BP 180 were detectable in 39 of 40 (98\%) of BP sera; interestingly, 88\% of BP sera also contained IgA anti-BP180 autoantibodies. Similarly, anit-BP180 reactivity in LAD sera (n=22) was also attributed to both an IgA (68\%) and an IgG (76\%) autoanitbody response. IgA and IgG autoantibodies to the intracellular portion of BP180 were found in 14\% and 28\% of BP sera, respectively, and in 8\% of LAD sera (same percentage for both isotypes). Our findings clearly demonstrate that both BP and LAD patients have dual IgA and IgG autoimmune response to BP180 which is directed not only to the ectodomain, but also to the intracellular portion of this protein. (C) 2000 Academic Press.",

author = "Arno Kromminga and Christian Scheckenbach and Matthias Georgi and Christine Hagel and R{\"u}diger Arndt and Enno Christophers and Br{\"o}cker, \{Eva B.\} and Detlef Zillikens",

note = "Funding Information: This work has been supported by grant 98.073.1 from the Wilhelm Sander-Stiftung, Munich, Germany (D.Z.). We thank Drs Gerald Messer and Karin Partscht, Munich, Germany, for providing us with serum samples from patients with linear IgA disease and Drs George J. Giudice and Luis A. Diaz, Milwaukee, WI, USA, for providing us with rabbit sera 136 and 594. We are also grateful to Christa Knaus, W{\"u}rzburg, for assistance producing LAD-1 from conditioned HaCaT cell medium, and Hartmut Wulf, Hamburg, for help preparing the figures.",

year = "2000",

doi = "10.1006/jaut.2000.0437",

language = "English",

volume = "15",

pages = "293--300",

journal = "Journal of Autoimmunity",

issn = "0896-8411",

publisher = "Academic Press",

number = "3",

}

TY - JOUR

T1 - Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180

AU - Kromminga, Arno

AU - Scheckenbach, Christian

AU - Georgi, Matthias

AU - Hagel, Christine

AU - Arndt, Rüdiger

AU - Christophers, Enno

AU - Bröcker, Eva B.

AU - Zillikens, Detlef

N1 - Funding Information: This work has been supported by grant 98.073.1 from the Wilhelm Sander-Stiftung, Munich, Germany (D.Z.). We thank Drs Gerald Messer and Karin Partscht, Munich, Germany, for providing us with serum samples from patients with linear IgA disease and Drs George J. Giudice and Luis A. Diaz, Milwaukee, WI, USA, for providing us with rabbit sera 136 and 594. We are also grateful to Christa Knaus, Würzburg, for assistance producing LAD-1 from conditioned HaCaT cell medium, and Hartmut Wulf, Hamburg, for help preparing the figures.

PY - 2000

Y1 - 2000

N2 - Bullous pemphigoid (BP) and linear IgA disease (LAD) are autoimmune subepidermal blistering skin disease associated with autoantibodies against the transmembrane hemidesmosomal protein BP180/type XVII collagen. It has been demonstrated previously that BP is characterized predominantly by IgG autoantibodies, while autoantibodies in LAD mainly belong to the IgA isotype. The aim of the present study was to investigate the hypothesis that there is a significant overlap in the autoantibody isotype associated with these two disease. Several new recombinant forms of BP180 were generated in the baculovirus expression system, including the full-length protein. IgG autoantibodies to BP 180 were detectable in 39 of 40 (98%) of BP sera; interestingly, 88% of BP sera also contained IgA anti-BP180 autoantibodies. Similarly, anit-BP180 reactivity in LAD sera (n=22) was also attributed to both an IgA (68%) and an IgG (76%) autoanitbody response. IgA and IgG autoantibodies to the intracellular portion of BP180 were found in 14% and 28% of BP sera, respectively, and in 8% of LAD sera (same percentage for both isotypes). Our findings clearly demonstrate that both BP and LAD patients have dual IgA and IgG autoimmune response to BP180 which is directed not only to the ectodomain, but also to the intracellular portion of this protein. (C) 2000 Academic Press.

AB - Bullous pemphigoid (BP) and linear IgA disease (LAD) are autoimmune subepidermal blistering skin disease associated with autoantibodies against the transmembrane hemidesmosomal protein BP180/type XVII collagen. It has been demonstrated previously that BP is characterized predominantly by IgG autoantibodies, while autoantibodies in LAD mainly belong to the IgA isotype. The aim of the present study was to investigate the hypothesis that there is a significant overlap in the autoantibody isotype associated with these two disease. Several new recombinant forms of BP180 were generated in the baculovirus expression system, including the full-length protein. IgG autoantibodies to BP 180 were detectable in 39 of 40 (98%) of BP sera; interestingly, 88% of BP sera also contained IgA anti-BP180 autoantibodies. Similarly, anit-BP180 reactivity in LAD sera (n=22) was also attributed to both an IgA (68%) and an IgG (76%) autoanitbody response. IgA and IgG autoantibodies to the intracellular portion of BP180 were found in 14% and 28% of BP sera, respectively, and in 8% of LAD sera (same percentage for both isotypes). Our findings clearly demonstrate that both BP and LAD patients have dual IgA and IgG autoimmune response to BP180 which is directed not only to the ectodomain, but also to the intracellular portion of this protein. (C) 2000 Academic Press.

UR - https://www.scopus.com/pages/publications/0033712589

U2 - 10.1006/jaut.2000.0437

DO - 10.1006/jaut.2000.0437

M3 - Journal articles

C2 - 11040070

AN - SCOPUS:0033712589

SN - 0896-8411

VL - 15

SP - 293

EP - 300

JO - Journal of Autoimmunity

JF - Journal of Autoimmunity

IS - 3

ER -

Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180

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