Pathogenesis of mucosal disease: A cytopathogenic pestivirus generated by an internal deletion

Norbert Tautz, Heinz Jürgen Thiel, Edward J. Dubovi, Gregor Meyers*

*Corresponding author for this work
86 Citations (Scopus)


Cytopathogenic bovine viral diarrhea virus (BVDV) arises by RNA recombination in animals persistently infected with noncytopathogenic BVDV. Such animals develop fatal mucosal disease. In this report, the genome of a cytopathogenic BVDV isolate, termed CP9, is characterized. CP9-infected cells contained not only viral genomic RNA of 12.3 kb but also a BVDV-specific RNA of 8 kb. cDNA cloning and sequencing revealed that the 8-kb RNA is a BVDV genome with an internal deletion of 4.3 kb. The 8-kb RNA represents the genome of a typical defective interfering particle (DI), since its replication was strictly dependent on the presence of a helper virus and strongly interfered with the replication of the helper. Cell culture experiments demonstrated that the CP9 virus stock contains two viruses, namely, a helper virus and DI9. While the helper virus alone was noncytopathogenic, the presence of the DI conferred cytopathogenicity. Expression experiments demonstrated that p80, the marker protein of cytopathogenic BVDV, is translated from the defective genome. The occurrence of this cytopathogenic DI is linked to a fatal disease in cattle.

Original languageEnglish
JournalJournal of Virology
Issue number5
Pages (from-to)3289-3297
Number of pages9
Publication statusPublished - 01.05.1994

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)


Dive into the research topics of 'Pathogenesis of mucosal disease: A cytopathogenic pestivirus generated by an internal deletion'. Together they form a unique fingerprint.

Cite this