TY - JOUR
T1 - p53 Status in radiation-induced soft-tissue sarcomas
AU - Taubert, Helge
AU - Meye, Axel
AU - Bache, Matthias
AU - Hinze, Raoul
AU - Holzhausen, Hans Jürgen
AU - Schmidt, Hannelore
AU - Rath, Friedrich Wilhelm
AU - Dunst, Jürgen
AU - Würl, Peter
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998/8
Y1 - 1998/8
N2 - Background: Following therapeutic irradiation after a latency period of many years radiation-induced tumors, often sarcomas, can arise. Results of radiation-induced DNA damage can be 1. p53 over-expression, inducing growth arrest or apoptosis, and 2. occurrence of mutations, frequently including the p53 gene, as one molecular promotor for carcinogenesis. We were interested whether radiation-induced sarcomas are associated with alterations of the p53 status. Material and Methods: Samples from 11 radiation-induced soft-tissue sarcomas (STS) were studied by a non-radioactive PCR-SSCP sequencing analysis and by immunohistochemistry with five antibodies for their p53 status. Results: A tumor of one patient possessed a G→A transition in codon 280 (exon 8). Of 11 tumors, 9 showed nuclear p53 positivity, detected by monoclonal antibody DO-1. Of these 9 patients, 7 died during the observation period, whereas the 2 patients with DO-1 negative tumor samples are still alive. Conclusions: p53 over-expression and p53 mutation occur in radiation- induced STS. p53 status is expected to have prognostic impact for radiation- induced STS.
AB - Background: Following therapeutic irradiation after a latency period of many years radiation-induced tumors, often sarcomas, can arise. Results of radiation-induced DNA damage can be 1. p53 over-expression, inducing growth arrest or apoptosis, and 2. occurrence of mutations, frequently including the p53 gene, as one molecular promotor for carcinogenesis. We were interested whether radiation-induced sarcomas are associated with alterations of the p53 status. Material and Methods: Samples from 11 radiation-induced soft-tissue sarcomas (STS) were studied by a non-radioactive PCR-SSCP sequencing analysis and by immunohistochemistry with five antibodies for their p53 status. Results: A tumor of one patient possessed a G→A transition in codon 280 (exon 8). Of 11 tumors, 9 showed nuclear p53 positivity, detected by monoclonal antibody DO-1. Of these 9 patients, 7 died during the observation period, whereas the 2 patients with DO-1 negative tumor samples are still alive. Conclusions: p53 over-expression and p53 mutation occur in radiation- induced STS. p53 status is expected to have prognostic impact for radiation- induced STS.
UR - http://www.scopus.com/inward/record.url?scp=0031814607&partnerID=8YFLogxK
U2 - 10.1007/BF03038565
DO - 10.1007/BF03038565
M3 - Journal articles
C2 - 9739384
AN - SCOPUS:0031814607
SN - 0179-7158
VL - 174
SP - 427
EP - 430
JO - Strahlentherapie und Onkologie
JF - Strahlentherapie und Onkologie
IS - 8
ER -