Oxygen binding and acid base status of the blood from the freshwater turtle, Phrynops hilarii

E. Reischl*, W. Jelkmann, K. H. Götz, C. Albers, C. Bauer

*Corresponding author for this work
4 Citations (Scopus)

Abstract

1. 1. Oxygenation studies with the whole blood of Phrynops hilarii show a P50 of 38 torr at extracellular pH (pHe) of 7.4 which corresponds to an intracellular pH (pHi) of 7.05 at 25°C. 2. 2. The blood CO2 Bohr effect was -0.56 when related to pHi. 3. 3. pHi is related to pHe by the following equation: pHi = 0.75.pHe + 1.54 (r = 0.99); pHi = 0.72.pHe + 1.72 (r = 0.96) at 10 and 25°C respectively. Blood pH, pHe, for 25°C, was 7.519 ± 0.254 (n = 6). 4. 4. Blood gas partial pressures were: pCO2 = 25.8 ± 3.8 torr (n = 6); pO2 = 61.7 ± 21.2 torr (n = 6). 5. 5. The major red cell phosphates, in mmole/l erythrocytes, n = 6, were: ATP (3.66 ± 0.86); GTP (0.53 ± 0.28); 2.3-DPG (0.32 ± 0.12) and inorganic phosphates (2.00 ± 0.35). 6. 6. The plasma inorganic ion composition, n = 6, was, in mEq/l: K+ (3.04 ± 0.40); Na+ (148.4 ± 12.6); Ca2+ (4.75 ± 1.32); Cl (106.6 ± 5.0). 7. 7. Additional blood parameters of interest (n = 6) were: lactate (2.07 ± 1.72 mM in plasma); erythrocytes/mm3 (416 × 103 ± 4.6 × 103); leucocytes/mm3 (4636 ± 2618); haematocrit (%) (14.5 ± 3.6); haemoglobin, g/dl (3.2 ± 0.5); plasma protein g/dl (4.4 ± 0.4); osmolarity (293 ± 10 mOsm/l). 8. 8. The non-bicarbonate buffer value was -22.6 mmol/kg H2O/pH. 9. 9. For a constant CO2 content, ΔpHe Δt = 0.0141 ± 0.002 (n = 18) and ΔpHi Δt = 0.0157 ± 0.003 (n = 18).

Original languageEnglish
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume78
Issue number2
Pages (from-to)443-446
Number of pages4
ISSN0305-0491
DOIs
Publication statusPublished - 1984

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

Fingerprint

Dive into the research topics of 'Oxygen binding and acid base status of the blood from the freshwater turtle, Phrynops hilarii'. Together they form a unique fingerprint.

Cite this