Optical Microscopy and Spectroscopy of Single Molecules

Christian Hübner*, Thomas Basché

*Corresponding author for this work


This chapter contains sections titled:


Photophysical Principles of Single‐Molecule Fluorescence Detection

The Single Molecule as a Three‐Level System

Dipole–Dipole Coupled Oscillators

Weak Coupling

Strong Coupling

Experimental Techniques

Signal‐to‐Noise Considerations

Room‐Temperature Single‐Molecule Spectroscopy

Epifluorescence Microscopy

Total Internal Reflection (TIR) Microscopy

Scanning Confocal Optical Microscopy

Two‐Photon‐, 4π‐ and STED Microscopy

Scanning Near‐Field Optical Microscopy

Single‐Molecule Spectroscopy at Cryogenic Temperatures

The Laser System


Photon Antibunching

Photon Bunching

Electronic Coupling Between Molecules

Single Molecules as Antennas: Orientation
Original languageEnglish
Title of host publicationPhotophysics of Molecular Materials: From Single Molecules to Single Crystals
Number of pages43
PublisherWiley-VCH Verlag GmbH & Co. KGaA
Publication date27.04.2006
ISBN (Print)9783527404568
Publication statusPublished - 27.04.2006


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