On-Chip Neo-Glycopeptide Synthesis for Multivalent Glycan Presentation

Marco Mende, Alexandra Tsouka, Jasmin Heidepriem, Grigori Paris, Daniela S. Mattes, Stephan Eickelmann, Vittorio Bordoni, Robert Wawrzinek, Felix F. Fuchsberger, Peter H. Seeberger, Christoph Rademacher, Martina Delbianco, Alvaro Mallagaray, Felix F. Loeffler*

*Corresponding author for this work
2 Citations (Scopus)


Single glycan–protein interactions are often weak, such that glycan binding partners commonly utilize multiple, spatially defined binding sites to enhance binding avidity and specificity. Current array technologies usually neglect defined multivalent display. Laser-based array synthesis technology allows for flexible and rapid on-surface synthesis of different peptides. By combining this technique with click chemistry, neo-glycopeptides were produced directly on a functionalized glass slide in the microarray format. Density and spatial distribution of carbohydrates can be tuned, resulting in well-defined glycan structures for multivalent display. The two lectins concanavalin A and langerin were probed with different glycans on multivalent scaffolds, revealing strong spacing-, density-, and ligand-dependent binding. In addition, we could also measure the surface dissociation constant. This approach allows for a rapid generation, screening, and optimization of a multitude of multivalent scaffolds for glycan binding.

Original languageEnglish
JournalChemistry - A European Journal
Issue number44
Pages (from-to)9954-9963
Number of pages10
Publication statusPublished - 06.08.2020

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)


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