TY - JOUR
T1 - Novel ELISA systems for antibodies to desmoglein 1 and 3
T2 - Correlation of disease activity with serum autoantibody levels in individual pemphigus patients
AU - Schmidt, Enno
AU - Dähnrich, Cornelia
AU - Rosemann, Anke
AU - Probst, Christian
AU - Komorowski, Lars
AU - Saschenbrecker, Sandra
AU - Schlumberger, Wolfgang
AU - Stöcker, Winfried
AU - Hashimoto, Takashi
AU - Bröcker, Eva Bettina
AU - Recke, Andreas
AU - Rose, Christian
AU - Zillikens, Detlef
PY - 2010/5/1
Y1 - 2010/5/1
N2 - Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are intraepidermal blistering skin diseases. PV is characterised by autoantibodies directed against desmoglein (Dsg) 3 and in patients with the mucocutaneous variant also against Dsg 1, whereas in PF, only Dsg 1 is targeted. Here, ectodomains of Dsg 3 and Dsg 1 were recombinantly expressed in a human cell line (HEK293) and applied as authentic solid phases in ELISA test systems. Autoantibodies against Dsg 3 and/or Dsg 1 could be detected in 71 (100%) of 71 PV sera and against Dsg 1 in 48 (96%) of 50 PF sera. Control sera showed reactivity with Dsg 3 and Dsg 1 in 0.2% and 0.7%, respectively, of 401 healthy blood donors and in 2.1% of 48 randomly selected patients with bullous pemphigoid. No reactivity with Dsg 1 and 3 was detected in 21 patients with linear IgA disease. For both pemphigus variants, a statistically significant correlation between clinical severity and autoantibody levels was observed as demonstrated for 10 PV and 5 PF patients. In conclusion, the use of the ectodomains of Dsg 3 and 1 as target antigens expressed in a human cell line resulted in sensitive and specific ELISA systems for both diagnosis and monitoring of PV and PF.
AB - Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are intraepidermal blistering skin diseases. PV is characterised by autoantibodies directed against desmoglein (Dsg) 3 and in patients with the mucocutaneous variant also against Dsg 1, whereas in PF, only Dsg 1 is targeted. Here, ectodomains of Dsg 3 and Dsg 1 were recombinantly expressed in a human cell line (HEK293) and applied as authentic solid phases in ELISA test systems. Autoantibodies against Dsg 3 and/or Dsg 1 could be detected in 71 (100%) of 71 PV sera and against Dsg 1 in 48 (96%) of 50 PF sera. Control sera showed reactivity with Dsg 3 and Dsg 1 in 0.2% and 0.7%, respectively, of 401 healthy blood donors and in 2.1% of 48 randomly selected patients with bullous pemphigoid. No reactivity with Dsg 1 and 3 was detected in 21 patients with linear IgA disease. For both pemphigus variants, a statistically significant correlation between clinical severity and autoantibody levels was observed as demonstrated for 10 PV and 5 PF patients. In conclusion, the use of the ectodomains of Dsg 3 and 1 as target antigens expressed in a human cell line resulted in sensitive and specific ELISA systems for both diagnosis and monitoring of PV and PF.
UR - http://www.scopus.com/inward/record.url?scp=77953166421&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0625.2010.01069.x
DO - 10.1111/j.1600-0625.2010.01069.x
M3 - Journal articles
C2 - 20163452
AN - SCOPUS:77953166421
SN - 0906-6705
VL - 19
SP - 458
EP - 463
JO - Experimental Dermatology
JF - Experimental Dermatology
IS - 5
ER -
