Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

Inga Kaufhold; Sünja Osbahr; Kensuke Shima; Sebastian Marwitz; Kristina Rohmann; Daniel Drömann; Torsten Goldmann; Klaus Dalhoff; Jan Rupp

doi:10.1016/j.micinf.2017.07.002

Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

Inga Kaufhold^*, Sünja Osbahr, Kensuke Shima, Sebastian Marwitz, Kristina Rohmann, Daniel Drömann, Torsten Goldmann, Klaus Dalhoff, Jan Rupp

^*Corresponding author for this work

12 Citations (Scopus)

Abstract

Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.

Original language	English
Journal	Microbes and Infection
Volume	19
Issue number	11
Pages (from-to)	560-566
Number of pages	7
ISSN	1286-4579
DOIs	https://doi.org/10.1016/j.micinf.2017.07.002
Publication status	Published - 01.11.2017

Funding

We thank Anke Hellberg and Kristin Wischnat (Clinic of Infectious Diseases and Microbiology, University of Lübeck) for excellent technical support and Christine Klein and Victor Krajka (Institute of Neurogenetics, University of Lübeck) for assistance with confocal microscopy. This work was supported in part by the BMBF/German Center of Infection Research and German Center for Lung Research.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1016/j.micinf.2017.07.002

Cite this

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title = "Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells",

abstract = "Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.",

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T1 - Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

AU - Kaufhold, Inga

AU - Osbahr, Sünja

AU - Shima, Kensuke

AU - Marwitz, Sebastian

AU - Rohmann, Kristina

AU - Drömann, Daniel

AU - Goldmann, Torsten

AU - Dalhoff, Klaus

AU - Rupp, Jan

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.

AB - Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.

UR - https://www.scopus.com/pages/publications/85027964181

U2 - 10.1016/j.micinf.2017.07.002

DO - 10.1016/j.micinf.2017.07.002

M3 - Journal articles

AN - SCOPUS:85027964181

SN - 1286-4579

VL - 19

SP - 560

EP - 566

JO - Microbes and Infection

JF - Microbes and Infection

IS - 11

ER -

Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

Abstract

Funding

UN SDGs

Research Areas and Centers

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