Non-photonic sensing of membrane-delimited reactive species with a Na+ channel protein containing selenocysteine

Navin K. Ojha; Enrico Leipold; Roland Schönherr; Toshinori Hoshi; Stefan H. Heinemann

doi:10.1038/srep46003

Non-photonic sensing of membrane-delimited reactive species with a Na+ channel protein containing selenocysteine

Navin K. Ojha, Enrico Leipold, Roland Schönherr, Toshinori Hoshi, Stefan H. Heinemann^*

^*Corresponding author for this work

Clinic of Anesthesiology

Abstract

Photonic experiments are of key importance in life sciences but light-induced side effects are serious confounding factors. Here we introduce roNaV2, an engineered voltage-gated Na+ channel harboring a selenocysteine in its inactivation motif, as a non-photonic, sensitive, gateable, and reversible sensor for membrane-delimited reactive species. roNaV2 allows for the assessment of chemical modification induced in fluorescence microscopy settings with high sensitivity and time resolution and it demonstrates the usefulness of ion channels as highly sensitive reporters of membrane processes.

Original language	English
Article number	46003
Journal	Scientific Reports
Volume	7
Pages (from-to)	46003
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep46003
Publication status	Published - 05.04.2017

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

DFG Research Classification Scheme

2.22-11 Anaesthesiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/srep46003

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AU - Ojha, Navin K.

AU - Leipold, Enrico

AU - Schönherr, Roland

AU - Hoshi, Toshinori

AU - Heinemann, Stefan H.

PY - 2017/4/5

Y1 - 2017/4/5

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AB - Photonic experiments are of key importance in life sciences but light-induced side effects are serious confounding factors. Here we introduce roNaV2, an engineered voltage-gated Na+ channel harboring a selenocysteine in its inactivation motif, as a non-photonic, sensitive, gateable, and reversible sensor for membrane-delimited reactive species. roNaV2 allows for the assessment of chemical modification induced in fluorescence microscopy settings with high sensitivity and time resolution and it demonstrates the usefulness of ion channels as highly sensitive reporters of membrane processes.

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Non-photonic sensing of membrane-delimited reactive species with a Na+ channel protein containing selenocysteine

Abstract

Research Areas and Centers

DFG Research Classification Scheme

UN SDGs

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