TY - JOUR
T1 - NGS-based methylation profiling differentiates TCF3-HLF and TCF3-PBX1 positive B-cell acute lymphoblastic leukemia
AU - Kachroo, Priyadarshini
AU - Szymczak, Silke
AU - Heinsen, Femke Anouska
AU - Forster, Michael
AU - Bethune, Jörn
AU - Hemmrich-Stanisak, Georg
AU - Baker, Lewis
AU - Schrappe, Martin
AU - Stanulla, Martin
AU - Franke, Andre
PY - 2018/2
Y1 - 2018/2
N2 - Aim: To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. Materials & methods: Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and after remission and analyzed using reduced representation bisulfite sequencing and RNA-sequencing. Results: We identified 7000 differentially methylated CpG sites between subtypes, of which 78% had lower methylation levels in TCF3-HLF. Gene expression was negatively correlated with CpG sites in 23 genes. KBTBD11 clearly differed in methylation and expression between subtypes and before and after remission in TCF3-HLF samples. Conclusion: KBTBD11 hypomethylation may be a promising potential target for further experimental validation especially for the TCF3-HLF subtype.
AB - Aim: To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. Materials & methods: Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and after remission and analyzed using reduced representation bisulfite sequencing and RNA-sequencing. Results: We identified 7000 differentially methylated CpG sites between subtypes, of which 78% had lower methylation levels in TCF3-HLF. Gene expression was negatively correlated with CpG sites in 23 genes. KBTBD11 clearly differed in methylation and expression between subtypes and before and after remission in TCF3-HLF samples. Conclusion: KBTBD11 hypomethylation may be a promising potential target for further experimental validation especially for the TCF3-HLF subtype.
UR - http://www.scopus.com/inward/record.url?scp=85041095766&partnerID=8YFLogxK
U2 - 10.2217/epi-2017-0080
DO - 10.2217/epi-2017-0080
M3 - Journal articles
C2 - 29334255
AN - SCOPUS:85041095766
SN - 1750-1911
VL - 10
SP - 133
EP - 147
JO - Epigenomics
JF - Epigenomics
IS - 2
ER -
