New nucleotide analogues with enhanced signal properties

Dmitry Cherkasov*, Thorsten Biet, Englbert Bäuml, Walther Traut, Michael Lohoff

*Corresponding author for this work
8 Citations (Scopus)

Abstract

We describe synthesis and testing of a novel type of dye-modified nucleotides which we call macromolecular nucleotides (m-Nucs). Macromolecular nucleotides comprise a nucleotide moiety, a macromolecular linear linker, and a large macromolecular ligand carrying multiple fluorescent dyes. With incorporation of the nucleotide moiety into the growing nucleic acid strand during enzymatic synthesis, the macromolecular ligand together with the coupled dyes is bound to the nucleic acid. By the use of this new class of modified nucleotides, signals from multiple dye molecules can be obtained after a single enzymatic incorporation event. The modified nucleotides are considered especially useful in the fields of nanobiotechnology, where signal stability and intensity is a limiting factor.

Original languageEnglish
JournalBioconjugate Chemistry
Volume21
Issue number1
Pages (from-to)122-129
Number of pages8
ISSN1043-1802
DOIs
Publication statusPublished - 20.01.2010

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