TY - JOUR
T1 - Nerve growth factor influences IgE-mediated human basophil activation: Functional properties and intracellular mechanisms compared with IL-3
AU - Gibbs, Bernhard F.
AU - Zillikens, Detlef
AU - Grabbe, Jürgen
N1 - Funding Information:
The authors express their thanks to Professor Holger Kirchner (Institute of Immunology and Transfusion Medicine, University of Lübeck) for supplying Buffy coats. This project was funded in part by a grant from the Forschungsförderung der Universität zu Lübeck (Grant N07-2002).
PY - 2005/4
Y1 - 2005/4
N2 - NGF and IL-3 play a unique role in supporting human basophil differentiation and mediator secretion. Their importance in allergic disease is underlined further by studies showing elevated levels of these factors in asthmatics. Here, we compared the abilities of IL-3 and NGF to stimulate basophil histamine, IL-4 or IL-13 release, either directly or in conjunction with IgE-dependent stimulation and assessed the intracellular signals responsible. Our results show that the ability of IL-3 and NGF to enhance IgE-dependent histamine release are similar. Both factors also potentiated IgE-dependent IL-13 secretion to a greater degree than the release of histamine or IL-4. At high concentrations (100 ng/ml), IL-3 and NGF alone were capable of releasing cytokines but little histamine. These abilities of IL-3 and NGF to modulate basophil activation were sensitive to blockade by specific inhibitors of PI 3-kinase, p38 MAPK and PLC, but not PKC, suggesting that their effects are mediated considerably by pathways comparable to IgE-dependent signalling.
AB - NGF and IL-3 play a unique role in supporting human basophil differentiation and mediator secretion. Their importance in allergic disease is underlined further by studies showing elevated levels of these factors in asthmatics. Here, we compared the abilities of IL-3 and NGF to stimulate basophil histamine, IL-4 or IL-13 release, either directly or in conjunction with IgE-dependent stimulation and assessed the intracellular signals responsible. Our results show that the ability of IL-3 and NGF to enhance IgE-dependent histamine release are similar. Both factors also potentiated IgE-dependent IL-13 secretion to a greater degree than the release of histamine or IL-4. At high concentrations (100 ng/ml), IL-3 and NGF alone were capable of releasing cytokines but little histamine. These abilities of IL-3 and NGF to modulate basophil activation were sensitive to blockade by specific inhibitors of PI 3-kinase, p38 MAPK and PLC, but not PKC, suggesting that their effects are mediated considerably by pathways comparable to IgE-dependent signalling.
UR - http://www.scopus.com/inward/record.url?scp=13844253425&partnerID=8YFLogxK
U2 - 10.1016/j.intimp.2004.12.004
DO - 10.1016/j.intimp.2004.12.004
M3 - Journal articles
C2 - 15710342
AN - SCOPUS:13844253425
SN - 1567-5769
VL - 5
SP - 735
EP - 747
JO - International Immunopharmacology
JF - International Immunopharmacology
IS - 4
ER -
