Mycobacterium szulgai als Posititivkontrolle zur Detektion von Kontaminationen beim Nachweis des Mycobacterium tuberculosis-Komplexes durch eine spezifische 16S-rDNA-PCR an FFPE-Material

Translated title of the contribution: Mycobacterium szulgai as positive control helps to detect contaminations in the detection of Mycobacterium tuberculosis-complex in formalin fixed, paraffin embedded tissues by 16SrDNA PCR

Torsten Goldmann, Doris Hillemann, Florian Maurer, Barbara Kalsdorf, Rosemarie Krupar, Florian Stellmacher*, Sven Perner

*Corresponding author for this work

Abstract

The detection of Mycobacterium tuberculosis complex DNA by PCR using formalin-fixed paraffin-embedded material has become an integral part of molecular-pathological diagnostics. We describe an approach that enables the detection of contamination by using Mycobacterium szulgai as a positive control, contributing to the reduction of false-positive results.

Translated title of the contributionMycobacterium szulgai as positive control helps to detect contaminations in the detection of Mycobacterium tuberculosis-complex in formalin fixed, paraffin embedded tissues by 16SrDNA PCR
Original languageGerman
JournalPathologe
Volume42
Issue number1
Pages (from-to)83-85
Number of pages3
ISSN0172-8113
DOIs
Publication statusPublished - 02.2021

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

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