Multiple site-specific cleavage of HIV RNA by transcribed ribozymes from shotgun-type trimming plasmid.

J. Ohkawa; N. Yuyama; Y. Takebe; S. Nisikawa; M. Homann; G. Sczakiel; K. Taira

Multiple site-specific cleavage of HIV RNA by transcribed ribozymes from shotgun-type trimming plasmid.

J. Ohkawa^*, N. Yuyama, Y. Takebe, S. Nisikawa, M. Homann, G. Sczakiel, K. Taira

^*Corresponding author for this work

Institute of Molecular Medicine

National Institute of Advanced Industrial Science and Technology

14 Citations (Scopus)

Abstract

We have constructed a multiple-ribozyme expression system (designated shotgun type expression system). This system made it possible to express multiple ribozymes without reducing of the activities of each ribozyme. By using this system, multiple ribozymes specific for five different target sites in HIV-1 RNA were transcribed; all of them functioned independently of the others, and each cleaved the RNA at the respective predetermined target site only. Transient HIV-1 infection assay revealed that this kind of construct can disarm AIDS virus up to 96%, at least in a cultured cell. This proves that our system is effective in vivo as well.

Original language	English
Journal	Nucleic acids symposium series
Issue number	29
Pages (from-to)	121-122
Number of pages	2
ISSN	0261-3166
Publication status	Published - 1993

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abstract = "We have constructed a multiple-ribozyme expression system (designated shotgun type expression system). This system made it possible to express multiple ribozymes without reducing of the activities of each ribozyme. By using this system, multiple ribozymes specific for five different target sites in HIV-1 RNA were transcribed; all of them functioned independently of the others, and each cleaved the RNA at the respective predetermined target site only. Transient HIV-1 infection assay revealed that this kind of construct can disarm AIDS virus up to 96%, at least in a cultured cell. This proves that our system is effective in vivo as well.",

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T1 - Multiple site-specific cleavage of HIV RNA by transcribed ribozymes from shotgun-type trimming plasmid.

AU - Ohkawa, J.

AU - Yuyama, N.

AU - Takebe, Y.

AU - Nisikawa, S.

AU - Homann, M.

AU - Sczakiel, G.

AU - Taira, K.

PY - 1993

Y1 - 1993

N2 - We have constructed a multiple-ribozyme expression system (designated shotgun type expression system). This system made it possible to express multiple ribozymes without reducing of the activities of each ribozyme. By using this system, multiple ribozymes specific for five different target sites in HIV-1 RNA were transcribed; all of them functioned independently of the others, and each cleaved the RNA at the respective predetermined target site only. Transient HIV-1 infection assay revealed that this kind of construct can disarm AIDS virus up to 96%, at least in a cultured cell. This proves that our system is effective in vivo as well.

AB - We have constructed a multiple-ribozyme expression system (designated shotgun type expression system). This system made it possible to express multiple ribozymes without reducing of the activities of each ribozyme. By using this system, multiple ribozymes specific for five different target sites in HIV-1 RNA were transcribed; all of them functioned independently of the others, and each cleaved the RNA at the respective predetermined target site only. Transient HIV-1 infection assay revealed that this kind of construct can disarm AIDS virus up to 96%, at least in a cultured cell. This proves that our system is effective in vivo as well.

UR - http://www.scopus.com/inward/record.url?scp=0027358304&partnerID=8YFLogxK

M3 - Journal articles

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AN - SCOPUS:0027358304

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SP - 121

EP - 122

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

IS - 29

ER -

Multiple site-specific cleavage of HIV RNA by transcribed ribozymes from shotgun-type trimming plasmid.

Abstract

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